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Transgenic cotton regenerated plant transplantation method

A technology of regenerated strains and transgenes, applied in botany equipment and methods, horticulture, applications, etc., can solve the problems of cumbersome steps and low survival rate, and achieve the effect of improving the survival rate

Inactive Publication Date: 2011-05-25
COTTON RES INST SHANXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims to solve the problems of low survival rate and cumbersome steps caused by high temperature and low humidity in the existing transgenic cotton regenerated plant transplanting method, and provides a transgenic cotton regenerated plant transplanting method

Method used

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  • Transgenic cotton regenerated plant transplantation method
  • Transgenic cotton regenerated plant transplantation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Open the bottle cap of the rooted seedlings of the transgenic cotton regenerated plants to harden the seedlings in the bottle for 2 days; then wash off the root culture medium of the regenerated plants after hardening, plant them in a container with sterilized vermiculite, and water them thoroughly at one time. Cultivate in an incubator with a humidity of 90% and a temperature of 22° C. for 15 days; move it into a greenhouse and cultivate it until the cotton bolling matures.

Embodiment 2

[0023] Open the bottle cap of the rooted seedlings of the transgenic cotton regenerated plants to harden the seedlings in the bottle for 3 days; then wash off the root medium of the regenerated plants after hardening, plant them in a container with sterilized vermiculite, and water them thoroughly once. Cultivate in an incubator with a humidity of 88% and a temperature of 20° C. for 10 days; move it into a greenhouse and cultivate it until the cotton bolling matures.

Embodiment 3

[0025] Open the bottle cap of the rooted seedlings of the transgenic cotton regenerated plants to harden the seedlings in the bottle for 3 days; then wash off the root culture medium of the regenerated plants after hardening, plant them in a container with sterilized vermiculite, and water them thoroughly at one time. Cultivate in an incubator with a humidity of 89% and a temperature of 21° C. for 13 days; move it into a greenhouse and cultivate it until the cotton bolling matures.

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Abstract

The invention discloses a transgenic cotton regenerated plant transplantation method, which solves the problems that the survival rate of the transgenic cotton regenerated plants transplanted by the traditional method is low and the steps are trivial. The method comprises the following steps that: 1) the cover of a bottle containing transgenic cotton regenerated plant seedlings with roots is opened for indoor seedling hardening in the bottle for 2-3 days; 2) culture medium on the roots of the transgenic cotton regenerated plants is washed, the transgenic cotton regenerated plants are planted in a container containing sterilized roseite and are watered thoroughly at one step, and the transgenic cotton regenerated plants are cultured in an incubator with humidity of 88-90 percent and temperature of 20-22 DEG C for 10-15 days; and 3) the transgenic cotton regenerated plants are transplanted into greenhouse with normal conditions and grow to be mature to open bolls. The invention adopts the low-temperature and high-humidity environments for culture, so the survival rate reaches 90-95 percent.

Description

technical field [0001] The invention relates to the technical field of transgenic cotton, in particular to a transgenic cotton regenerated strain transplanting method. Background technique [0002] The transgenic regenerated strains obtained from cotton transformed with exogenous genes through Agrobacterium-mediated transformation, molecular testing proved that the exogenous genes had been transferred into the recipient cotton, and the transformed regenerated strains survived normally by grafting or conventional transplanting. Then grow into the desired transgenic cotton. [0003] The conventional transplanting method of transgenic cotton regenerated strains is as follows: first, open the cap of the bottle cap and place the rooted seedlings of the transgenic cotton regenerated plants in the triangular bottle for hardening for 1-2 days; In the container of vermiculite, pour water once and for all; then put it into an incubator with a humidity of 70% and a temperature of 27°C...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00
Inventor 李燕娥上官小霞吴霞张林水范小平杨淑巧翟晓菊
Owner COTTON RES INST SHANXI ACAD OF AGRI SCI