Method for detecting human lactoferrin content in human milk
A technology for the detection of human lactoferrin and content, which is applied in the detection field, can solve the undeveloped detection of human lactoferrin content and other problems, and achieve the effect of improving repeatability, high detection rate, and increasing safety
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Embodiment 1
[0025] The method for detecting human lactoferrin content in human milk of the present invention comprises the following steps:
[0026] (a) Dissolving the human lactoferrin standard substance in the sample buffer solution and preparing the standard solutions whose concentrations are respectively 4mg / ml, 2mg / ml, 1mg / ml, 0.5mg / ml and 0.25mg / ml, the human milk Put the ferritin standard solution in a centrifuge tube, shake it up, add it to the sample tube, clean it with ultrasonic waves, and use a high-pressure capillary electrophoresis analyzer for detection and analysis after shaking it up. 352612, 175234, 86524, according to the relationship between the concentration of the above standard solution and the corresponding peak area, use the software to draw the standard curve, the linear regression correlation coefficient of the standard curve obtained is 0.9974, the lowest detection limit is 0.1ug, the lowest The detected content is 0.01mg / ml, and the standard sample recovery ra...
Embodiment 2
[0033] The method for detecting human lactoferrin content in human milk of the present invention comprises the following steps:
[0034] (a) Dissolving the human lactoferrin standard substance in the sample buffer solution and preparing the standard solutions whose concentrations are respectively 4mg / ml, 2mg / ml, 1mg / ml, 0.5mg / ml and 0.25mg / ml, the human milk Put the ferritin standard solution in a centrifuge tube, shake it up, add it to the sample tube, clean it with ultrasonic waves, and use a high-pressure capillary electrophoresis analyzer for detection and analysis after shaking it up. 325687, 163518, 79685, according to the relationship between the concentration of the above standard solution and the corresponding peak area, use the software to draw the standard curve, the linear regression correlation coefficient of the standard curve obtained is 0.9997, the lowest detection limit is 0.002ug, the lowest The detected content is 0.01mg / ml, and the standard sample recovery ...
Embodiment 3
[0041] The method for detecting human lactoferrin content in human milk of the present invention comprises the following steps:
[0042] (a) the human lactoferrin standard substance is dissolved in the sample buffer solution and the preparation concentration is respectively the standard solution of 8mg / ml, 4mg / ml, 2mg / ml, 1mg / ml, 0.01mg / ml, the human lactoferrin Put the protein standard solution in a centrifuge tube, shake it up and add it to the sample tube, clean it with ultrasonic waves, shake it up and use a high-pressure capillary electrophoresis analyzer for detection and analysis. , 314385, 3124, according to the relationship between the concentration of the above standard solution and the corresponding peak area, use the software to draw the standard curve, the linear regression correlation coefficient of the standard curve obtained is 0.9969, the minimum detection limit is 2ng, the minimum detection The content is 0.01mg / ml, and the standard sample recovery rate is 92...
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