Acipenser baerii ovum short-term preserving fluid, preparation method thereof and use thereof

Abstract

The invention relates to acipenser baerii ovum short-term preserving fluid. One liter of the fluid comprises the following components: 120 mmol of sodium chloride, 5.67 mmol of potassium chloride, 0.78 mmol of magnesium sulfate, 0.65 mmol of calcium chloride, 4.24 mmol of glucose, 20 mmol of sodium bicarbonate, 1 gram of bovine serum albumin, 20 mmol of HEPES buffer salt, 200,000 IU of penicillin and 0.2 gram of streptomycin; and the pH value of the fluid is 8.0. The preparation method comprises: at normal temperature, preparing the preserving fluid by using the components; and adjusting the pH value of the fluid to 8.0 with 1mmol / L solution of NaOH. The acipenser baerii ovum short-term preserving fluid can realize the short-term preservation of acipenser baerii ova, namely preserving the ova at 16 DEG C for 4 hours for achieving a fertility rate of 86.36 percent, a hatching rate of 94.74 percent and an aberration rate of 0, and solves the technical problem caused by the asynchronous spawning inducing effects of female and male acipenser baerii.

Description

technical field The invention belongs to the field of aquatic biological ovum preservation solution and its preparation and application, and in particular relates to a short-term preservation solution of Siberian sturgeon ovum and its preparation and application. Background technique Siberian sturgeon is the main economic aquaculture species in my country. It has the advantages of fast growth, strong adaptability, good meat quality, and high-quality caviar. Since it was first introduced into China in 1996, it has developed into a breeding output second only to Sturgeon's sturgeon. the second largest cultured species of sturgeon. Once the eggs of fish are discharged, their viability will decline rapidly with the extension of time. After the eggs are discharged, choosing and using the best storage time is very important to improve the viability of the eggs. The eggs must be discharged within a certain period of time. Fertilization can ensure the normal development of embryos....

AI Technical Summary

Problems solved by technology

The research on the short-term preservation of fish eggs mainly focuses on salmon trout and Cypriniformes, but there is no report on the short-term preservation of Siberian sturgeon eggs using the preservation solution at home and abroad

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