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Preparation method of sericin/hydroxyapatite composite porous bracket

A hydroxyapatite and sericin technology, applied in medical science, prosthesis and other directions, can solve the problems of insufficient mechanical properties, limited use, slow degradation, etc., and achieve the effects of simple preparation process, low cost and high porosity

Inactive Publication Date: 2013-01-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also disadvantages such as high brittleness, poor toughness, insufficient mechanical properties, slow degradation and easy migration from the implantation site, which limit its use as a human material implant.

Method used

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  • Preparation method of sericin/hydroxyapatite composite porous bracket
  • Preparation method of sericin/hydroxyapatite composite porous bracket
  • Preparation method of sericin/hydroxyapatite composite porous bracket

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: the preparation method of sericin solution, comprises the following steps:

[0037] (1) Put the silkworm cocoon layer after pupa removal into boiling water for degumming; the amount of water should be able to submerge the cocoon layer;

[0038] (2) After 30 minutes, stop cooking and filter to remove impurities;

[0039] (3) putting the filtered sericin solution into a rotary evaporator to concentrate to obtain the sericin solution of the target concentration;

[0040] (4) Extract the sericin solution, perform SDS gel electrophoresis, and measure the molecular weight of the sericin protein. The molecular weight range of the sericin protein is 97.2-200.0 KDa.

Embodiment 2

[0041] Embodiment 2, the preparation method of hydroxyapatite powder, comprises the following steps:

[0042] (1) Take 700ml saturated Ca(OH) 2 solution, placed in a water bath at 60°C, and added dropwise with 10%wt H 3 PO 4 Solution 11ml, during this period, use a magnetic stirrer to continuously and rapidly stir and keep the water temperature;

[0043] (2) Use a digital display pH meter to monitor the pH of the solution, and adjust the pH of the solution to remain at 8.7 by adding ammonia water dropwise;

[0044] (3) After adding H dropwise 3 PO 4 After the solution, continue to stir at a faster speed for 3 hours, and after the stirring is completed, place it at room temperature for 48 hours;

[0045] (4) incline the supernatant, and collect the lower part of the precipitate; the precipitate is first washed with absolute ethanol for 2-3 times, and then cleaned with distilled water for 3-4 times;

[0046] (5) Put the obtained precipitate into a refrigerator to freeze, t...

Embodiment 3

[0047] Embodiment 3, a preparation method of sericin / hydroxyapatite composite porous scaffold, comprising the following steps:

[0048] (1) Measure a certain amount of sericin solution and place it in a beaker, add hydroxyapatite powder therein in proportion to keep the ratio of the total amount of sericin protein and hydroxyapatite in the solution consistent;

[0049] (2) Put the mixed solution of sericin and hydroxyapatite into an ultrasonic cleaner, and vibrate for 3 to 5 minutes;

[0050] (3) Take out the mixed solution, slowly dropwise add a small amount of glutaraldehyde crosslinking agent with a concentration of 0.5%, and stir slowly with a magnetic stirrer;

[0051] (4) Quickly transfer the mixed solution into a 96-well cell culture plate, and immediately put it into a -20°C refrigerator to pre-freeze for 12 hours;

[0052] (5) Take out the plate sample after freezing for 12 hours, and carry out the temperature recovery treatment at room temperature of 20°C for 4 hour...

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Abstract

The invention discloses a preparation method of a sericin / hydroxyapatite composite porous bracket. The method comprises the following steps: respectively synthesizing hydroxyapatite powder and preparing a sericin solution, mixing according to different mass proportions, and using the freeze-drying method to prepare a sericin / hydroxyapatite composite porous bracket material. The prepared sericin / hydroxyapatite composite porous bracket material has the advantages of simple preparation process, short preparation time, easily-satisfied preparation conditions, no porogenic agent addition, less andcheap reagent and low cost. Besides, the prepared bracket material has higher porosity and mechanical property, and the pores are uniformly dispersed and are communicated mutually.

Description

technical field [0001] The invention relates to the field of processing and utilization of sericin, in particular to a preparation method of sericin / hydroxyapatite composite porous scaffold material in bone tissue engineering. Background technique [0002] Bone injury and bone defect are very common diseases in daily life. Traditional medicine mainly uses autologous and allograft transplantation to repair bone defects, but this method has disadvantages such as new damage and immune rejection, and is not a rational bone repair method. Bone tissue engineering is to use a small amount of tissue cells to repair bone defects after in vitro culture and expansion. At the same time, it can be arbitrarily shaped according to the bone defect to achieve perfect shape repair, which has opened up a new way to achieve non-damage repair. [0003] Sericin (Silk Sericin referred to as SS) is a globular protein with a relative molecular weight of 14,000 to 314,000 and composed of 18 amino ac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/12A61L27/56
Inventor 朱良均王琳婷杨明英闵思佳张海萍
Owner ZHEJIANG UNIV
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