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White rot fungus rot agent and method for producing organic pollutant rot agent by using white rot fungi

A technology of organic pollutants and white-rot bacteria, which is applied in the fields of bacteria, processing wastewater treatment, food industry wastewater treatment, etc., can solve the problems of algal bloom and fish death, environmental water pollution, affecting aircraft flight, etc., and achieves high economic efficiency. Value, good effect, professional effect

Inactive Publication Date: 2012-01-25
NANJING UNIV OF INFORMATION SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At the same time, there are thousands of livestock and poultry farms in my country, which produce billions of tons of livestock and poultry manure every year. Except for a part of which is used as organic fertilizer and applied to farmland, many of them are discharged into the environment without treatment, and eventually pollute the water body, causing algal blooms and Fish die and losses are high
[0006] Every year during the summer and autumn harvests, a large amount of crop straw is produced, which is difficult to dispose of. Farmers burn the environment and pollute the flight of aircraft, discard river water, block river courses, and deteriorate water quality. Wastewater from paper mills contains a large amount of lignin, causing serious environmental and environmental damage. water pollution

Method used

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  • White rot fungus rot agent and method for producing organic pollutant rot agent by using white rot fungi
  • White rot fungus rot agent and method for producing organic pollutant rot agent by using white rot fungi
  • White rot fungus rot agent and method for producing organic pollutant rot agent by using white rot fungi

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Effect test

example 1

[0032] Example 1: Solid culture method

[0033] 1. Thoroughly disinfect the culture environment, including culture tanks, curved discs, plastic bags, culture rooms and the air in the culture room.

[0034] 2. Mix 5-20% wheat bran, 5-20% rice bran, 30-50% cottonseed hull, 0.5-6% bean cake powder, 0.5-8% wheat flour, 0.1-3% ammonium sulfate, 0-0.4% dihydrogen phosphate Potassium, 0-0.3% magnesium sulfate, add appropriate amount of tap water, adjust the water content to about 40-50%, pH5-6.5, make a solid medium, put the solid medium in a culture tank, a curved plate or a plastic bag.

[0035] 3. Place the bagged raw materials in an autoclave for high-temperature sterilization for 60-70 minutes, and keep the temperature in the autoclave at 121° C. and the pressure at 0.15 MPa.

[0036] 4. Take out the sterilized bagged culture medium, place it in a sterile inoculation room to cool down, and place it for 2-3 days.

[0037] 5. Sterilize the sterilized bagged medium that has been ...

Embodiment 2

[0042] 1. Add appropriate amount of water to 15kg wheat bran, 15kg rice bran, 45kg cottonseed hulls, 3kg bean cake powder, 4.8kg wheat flour, 2kg ammonium sulfate, 0.1kg dipotassium hydrogen phosphate, 0.1kg magnesium sulfate, etc., and adjust the water content to 40-50 %, pH5-6.5, made into a solid culture medium, with a total weight (water content) of 127.5kg, and the solid culture medium was placed in a culture tank, a bent plate or a plastic bag.

[0043] 3. Place the bagged raw materials in an autoclave for high-temperature sterilization for 60-70 minutes, and keep the temperature in the autoclave at 121° C. and the pressure at 0.15 MPa.

[0044] 4. Take out the sterilized bagged culture medium, place it in a sterile inoculation room to cool down, and place it for 2-3 days.

[0045] 5. Sterilize the sterilized bagged medium that has been placed for 2-3 days at high temperature again, the method is the same as 3, kill the spores that survived the previous sterilization proce...

Embodiment 3

[0050] The white rot fungus product cultivated by the present invention is used as a paper mill sewage treatment agent. In the laboratory, 25 grams of white rot fungus preparations are added to the powder of this product per liter of waste water, and the stirring shaft is started to stir the sewage for aeration and cultivation. After 3 days, the sewage in the sewage The lignin degradation rate is above 64.5% (see figure 1 ).

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Abstract

The invention provides a white rot fungus rot agent and a method for producing an organic pollutant rot agent by using white rot fungi. In the method, the white rot fungi are obtained in a large quantity through industrial production and used as a treating agent for waste water, sewages and excrements of paper mills, textile mills, printing and dyeing mills, food factories, livestock and poultry factories, and the like, can also be used as a quick rot agent of composts prepared from farmland straws applied to farmlands and straw pollutions and has higher economic values and good application prospects. The invention also provides an application of the white rot fungi used as a waste water treating agent. The production method of the white rot fungi comprises the following steps of: puttinga culture medium in a culture container; after sterilization at a proper temperature, inoculating the white rot fungi to the culture medium and controlling the temperature, the humidity, the pressureand the pH condition of the culture medium; introducing sterile air; adsorbing by using attapulgite, peat, kaolin, and the like after culturing; drying by air or drying in the shade; and pulverizing to prepare into a solid or powdered finished product.

Description

Technical field: [0001] The present invention relates to a production method of a white rot fungus preparation, and the application of the preparation as an environmental treatment agent, mainly as a waste water treatment agent for paper mills, food factories, manure and sewage treatment agents for livestock and poultry farms, and for decomposing crop straws. Applications. Background technique: [0002] White rot fungus is a white filamentous fungus that makes wood decay white. Taxonomically, white rot fungi belong to the fungal phylum, mainly Basidiomycetes, and a few are Ascomycetes, with rich diversity. Compared with decomposing cellulose components, white rot fungi are more likely to degrade lignin. During the process of decomposing rotting lignin, they also decompose other polysaccharides. Under certain conditions, they can decompose the main components of wood and crop straw (lignin, cellulose) , hemicellulose) are all degraded to CO 2 and H 2 O, to complete the bi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F103/20C02F103/28C12N1/20C02F103/32
Inventor 吴洪生
Owner NANJING UNIV OF INFORMATION SCI & TECH