Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Listeria monocytogenes strains and uses thereof

A technique for monocytogenes, Listeria, applied in the field of bacteriology, which can solve problems such as lack of strains

Inactive Publication Date: 2011-12-21
NORTHEAST AGRICULTURAL UNIVERSITY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Only in this way can it provide a theoretical basis for preventing the spread of its drug resistance and the development of new antibacterial drugs. The main reason why the work in this area has not been carried out in depth is the lack of suitable strains for such research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Listeria monocytogenes strains and uses thereof
  • Listeria monocytogenes strains and uses thereof
  • Listeria monocytogenes strains and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The isolation and identification of embodiment 1 bacterial strain of the present invention

[0025] 1. Test materials

[0026] 1 Sample source Fresh pork was collected from major supermarkets and bazaars in Harbin.

[0027] 2 The standard strain Listeria monocytogenes CMCC54006 was purchased from China Medical Bacteria Collection Center.

[0028] 3 medium LB 1 , LB 2 Bacteria enrichment solution and SIM power semi-solid medium were purchased from Beijing Luqiao Biological Company, Komagalister chromogenic medium was purchased from Zhengzhou Bosai Bioengineering Co., Ltd., TSA-YE solid medium, TSB-YE liquid culture The base was purchased from Qingdao Haibo Biotechnology Co., Ltd., and the blood plate was made by the bacterial laboratory of Harbin Veterinary Research Institute.

[0029] 4 Primers Listeria monocytogenes-specific primers (primers for detecting the hemolysin gene hlyf) were synthesized by Jinsite Technology (Nanjing) Co., Ltd. The primer sequences are a...

Embodiment 2

[0042] The drug resistance spectrum analysis of embodiment 2 bacterial strains of the present invention

[0043] 1. Test materials

[0044] All the test drug-sensitive tablets were purchased from Beijing Tiantan Drug Biotechnology Development Company.

[0045] 2. Test method

[0046] The D38 strain isolated in Example 1 of the present invention was inoculated into TSB-YE medium, cultured at 37°C for 18 hours, washed with sterilized physiological saline, and the bacterial solution was diluted to 0.5 McFarland turbidity, and the concentration of the bacterial solution was corrected in The inoculation was completed within 15 minutes. Use a sterilized cotton swab to dip in the bacterial solution, rotate and squeeze out the excess bacterial solution on the inner wall of the tube, and then evenly spread and inoculate on the TSA-YE plate for 3 times, rotate the plate 60° each time, and finally spread a circle along the edge of the plate. Put the cover on and let it sit for 5 minut...

Embodiment 3

[0050] Embodiment 3 Determination of the tetracycline drug-resistant gene of bacterial strain of the present invention

[0051] 1. Test materials

[0052] 1 Reagents: DNA Marker and Ex Taq enzymes were purchased from Dalian Bao Biological Engineering Co., Ltd.

[0053] 2 Primers: TetM-specific primers for the tetracycline resistance gene were synthesized by Jinsite Technology (Nanjing) Co., Ltd. The primer sequences are as follows:

[0054] tetm-U 5′-ACAGAAAGCTTTATTATAAC-3′

[0055] tetm-L 5′-TGGCGTGTCTATGATGTTCAC-3′

[0056] 2. Test method

[0057] The D38 strain of the present invention was inoculated in TSB-YE medium, and after culturing at 37° C. for 24 hours, the genome of the strain was extracted with a genomic DNA extraction kit. Then the tetracycline resistance gene tetM was amplified by PCR under the reaction conditions of 95°C pre-denaturation for 5 min, 94° denaturation for 30 s, 50° annealing for 30 s, 72° extension for 1 min, 30 cycles, and 72° final extensio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a strain of Listeria monocytogenes and its application. The microorganism preservation number of the Listeria monocytogenes strain (Listeria monocytogenes) of the present invention is CGMCCNo.3342. The strain of the present invention is resistant to four antibiotics, fosfomycin, tetracycline, norfloxacin and cefotaxime at the same time, and is a quadruple drug-resistant strain, which can be used for the research of single drug resistance mechanism and multiple drug resistance mechanism, and can also be used for A study of drug resistance elimination assays. The bacterial strain of the present invention is a special bacterial strain carrying a transferable tetM gene, which can be used for the study of the horizontal transmission mechanism of the tetM drug resistance gene, and also provides a material basis for the study of prevention and control means for the spread of tetracycline drug resistance.

Description

technical field [0001] The invention relates to a small Gram-positive bacillus, in particular to a strain of Listeria monocytogenes and its use, and belongs to the field of bacteriology. Background technique [0002] Listeria monocytogenes (Listeria monocytogenes, L.M.) is a small Gram-positive bacillus. It widely exists in soil, water, insects, plants and animals. Food is the main carrier of the pathogenic bacteria. According to WHO (1999) reports, 4% to 8% of aquatic products, 5% to 10% of milk and dairy products, more than 30% of meat and meat products, and more than 15% of poultry are all contaminated by this bacteria. Because the bacteria can still grow and reproduce at 4°C, it has also become one of the main pathogenic bacteria that threaten human health in refrigerated food (Shen Hong, Jiang Xingxiang, Zhao Xiayun, etc. Listeria monocytogenes in food Detection and pollution status analysis [J], Chinese Journal of Zoonoses, 2007, 23(4): 417.). [0003] As one of th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/68C12Q1/02C12R1/01
Inventor 邵美丽韩建春邵红刘思国尹录边亚娟王亚君张秋佳
Owner NORTHEAST AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com