Sequence for expressing targeted double-recombinant apoptosis proteins of liver cancer and application thereof

Abstract

The invention provides a sequence for expressing targeted double-recombinant apoptosis proteins of liver cancer, which is a DNA sequence for regulating the expression of two apoptosis proteins of Caspase 3 and Granzyme B by using a specific AFP promoter, and has a nucleotide sequence shown as SEQID No:1. The sequence utilizes the function of targeted regulation of an AFP enhancer/promoter transcriptional regulatory element to lead foreign gene to be expressed in liver cancer cells with positive AFP, thus realizing targeted and high-efficiency anti-tumor effect; and the sequence constructs recombinant activated type Caspase-3 molecule, applies the recombinant activated type Caspase-3 to pro-apoptotic effect for target cells, introduces granzyme B, fuses the granzyme B and the recombinant activated type Caspase-3 molecule, and mediates the apoptosis of the target cells. The invention also provides an application in preparing targeted gene drugs for treating the liver caner with the positive AFP.

Description

technical field The present invention belongs to biological technology, and relates to the regulation of gene expression, in particular, to the design and preparation of DNA expressing a recombinant apoptosis-promoting protein specific for alpha-fetoprotein-positive liver cancer and its application in alpha-fetoprotein-positive liver cancer cells. Specifically expressed, its product can effectively induce the apoptosis of liver cancer cells. The invention can be used to prepare targeted tumor gene therapy drugs. Background technique Replication-defective adenovirus is the most classic form of adenovirus vector, it is a kind of adenovirus that cannot proliferate in cells, it only acts as a transport carrier, and can carry foreign genes into cells. The expression products of exogenous genes affect the growth of cells, thus playing the role of gene therapy. According to the mechanism of action of replication-defective adenovirus, in order to improve its therapeutic effect, it...

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Problems solved by technology

Although the third-generation adenovirus has obvious advantages, its production process is a major factor that hinders it

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