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Composition with anti-tumor effect and application thereof in preparing medicament for treating tumor

A technology of anti-tumor effect and composition, applied in the field of medicine, can solve the problem of fatal cardiotoxicity of toad venom and exacerbate the cardiotoxicity of toad venom, and achieve strong inhibitory effect, safe use, and low cardiotoxicity

Inactive Publication Date: 2010-09-08
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 中国专利,申请号为02135923.7、02135926.1、02114028.6、01128204.5、01128205.3、92102701.X、93104343.3、94105789.5、94114881.5、94110281.5、96121433.3、98114410.1、98100693.0、97111973.2、00110369.5、00110461.6、98112624.3、99121442.0、98125018.1、2007101984718、200710193175.9、 200810050571.0、200310116881.5、03150213.X、02144901.5、200510071081.5、200610042484.1、200610066458.2、200610018348.9、200610043412.9、200810107563.5的专利申请有公开含有蟾酥药材的抗肿瘤组合物,但是上述专利保护了含有多达几十余味药材为特征 The large prescription for tumor diseases does not solve the fatal cardiotoxicity problem caused by the active and toxic toad venom in the prescription, especially the large amount of other toxic drugs used in the above-mentioned patent prescription, such as: horse money Seedlings, garcinia cambogia, mylabris, realgar, etc. can further aggravate the cardiotoxicity of toad venom

Method used

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  • Composition with anti-tumor effect and application thereof in preparing medicament for treating tumor
  • Composition with anti-tumor effect and application thereof in preparing medicament for treating tumor
  • Composition with anti-tumor effect and application thereof in preparing medicament for treating tumor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Inhibitory activity of the combination of toad venom and attenuated adjuvant extracts on the proliferation of various tumor cells

[0032] MTT method was used to detect the effects of toad venom and the combination of toad venom and different drugs on human gastric cancer cell MGC803, human liver cancer cell SMMC7721, human lung cancer cell A549, human leukemia cell HL-60, human cervical cancer cell SiHa, human ovarian cancer cell A2780, human breast cancer cell The effect on the proliferation of cancer cell MCF-7 and human prostate cancer cell PC-3. Dimethyl sulfoxide was used as the negative control group, and paclitaxel was used as the positive control group. The specific experimental results are shown in Table 1 and Table 2.

[0033] Table 1 The inhibitory effect of the composition of Bufoni and different drugs on tumor cell proliferation

[0034]

[0035] Table 2 The inhibitory effect of the composition of Bufoni and different drugs on tumor cell proli...

Embodiment 2

[0039] Example 2 Inhibitory activity of bufasterene on the proliferation of various tumor cells after being combined with different drugs

[0040] MTT method was used to detect bufosterenes (lipobufagenin, cinobufacine, bufalin, hydroxycinobufagin, bufobufalin, yuanhuabufajing, deacetylated cinobufagin, toad The mixture of talinin, bufagin, hellebore aglycone, the same below) and bufastenene and different drugs have effects on human gastric cancer cell MGC803, human liver cancer cell SMMC7721, human lung cancer cell A549, human leukemia cell HL- 60. Effects on the proliferation of human cervical cancer cell SiHa, human ovarian cancer cell A2780, human breast cancer cell MCF-7, and human prostate cancer cell PC-3, with dimethyl sulfoxide as a negative control group and paclitaxel as a positive control group, and the specific experimental results are shown in Table 3.

[0041] Table 3 Inhibitory effect of the composition of bufasterene and different drugs on tumor cell prolifer...

Embodiment 3

[0044] Example 3 Inhibitory effect of the composition of toad and Chinese medicine compound on H22 tumor-bearing mice

[0045] Take 60 mice and divide them into 6 groups, 10 in each group. Each mouse was inoculated with 0.2ml of H22 tumor-bearing dilution, and then given Chansu 25mg / kg, Chansu + Danggui Buxue Decoction 25+600mg / kg, Chansu + Renshen Shaoyao Decoction 25 + 1600mg / kg, Chansu + Shengmai Decoction 25 + 500mg / kg, Chansu + Fumai Decoction 25 + 1000mg / kg, after seven days of continuous administration, the mice were killed to strip off the H22 tumor-bearing mice in each group, The tumor weight was weighed, and the tumor inhibition rate was calculated. The specific experimental results are shown in Table 4.

[0046] Table 4. The composition of Chansu and traditional Chinese medicine compound inhibits the tumor of H22 tumor-bearing mice

[0047]

[0048] The above experimental results show that the toad provided by the present invention is combined with the tr...

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Abstract

The invention provides a composition with anti-tumor effect and application thereof. The composition consists of 0.01 to 10 weight parts of pure bufadienolide compound, extract rich in bufadienolide compound, raw toad venom or toad skin and 0.01 to 99.9 weight parts of medicament with anti-arrhythmic effect. The composition with the anti-tumor effect has the advantage that the match among the components is scientific and reasonable; multiple in vitro tumor cytotoxicity and in vivo tumor inhibiting experiments show that the composition provided by the invention has good anti-tumor effect and plays a role in synergy after combination; and cardiotoxic experiments show that the cardiotoxicity of the composition provided by the invention is obviously reduced, and the composition can reduce the cardiotoxicity caused by primary single use of the bufadienolide compound, the toad venom or the toad skin and plays a role in detoxifying. Therefore, the composition is safer and more effective to use, and is expected to develop a new generation anti-cancer medicament.

Description

technical field [0001] The invention relates to a medicine, and specifically designs an antitumor composition with bufasteroids as active components and the application of the composition in preparing medicines for treating tumors. Background technique [0002] Malignant tumor is a disease with a high mortality rate. According to statistics, there are 2 million new cancer cases in my country every year, and 1.4 million deaths due to cancer. Among the 5 deaths of Chinese residents, 1 person died of cancer. At present, the main methods of tumor treatment include surgery, radiotherapy, chemotherapy, biological therapy, and endocrine therapy, etc., but Western medicine treatment has serious adverse reactions and is expensive. [0003] Toad Su, a traditional Chinese medicine, is a white slurry secreted by the back-auricular glands and skin glands of the bufo family Bufo bufo or black-orbited toad, which is processed and dried. Toad skin is the skin of Bufoidae animals, such as t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K35/56A61K31/585A61P35/00A61K35/65
Inventor 马宏跃
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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