Diagnostic reagent for detecting cow subclinical mastitis and preparation method thereof

A technology of subclinical mastitis and diagnostic reagents, applied in the field of veterinary inspection, can solve the problems of high toxicity, etc., and achieve the effects of stable diluent, stable product and high diagnostic sensitivity

Inactive Publication Date: 2010-09-08
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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[0031] The diagnostic coincidence rate test of the present invention

[0032] 1 test material

[0033] Diagnostic solution (prepared and diluted according to the aforementioned method), CMT diagnostic solution (imported from British COX), milk cell counter (Bently, USA), binocular biological microscope (domestic XSZ-107), glass slide, Loefflers methylene blue stain liquid, xylene, 95% ethanol.

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Abstract

The invention relates to a diagnostic reagent for detecting subclinical mastitis of mammals such as cows, sheep, pigs and the like and a preparation method for the reagent. The detection reagent is formed by the following components by mass percent: 8.8-9.2 percent of lauryl sodium sulfate, 4.8-5.2 percent of linear alkyl benzene sulfonic acid, 0.5-0.6 percent of boric acid, 0.04-0.08 percent of bromcresol purple, 0.6-0.8 percent of sodium hydroxide and remaining percent of water. The preparation method is that lauryl sodium sulfate, linear alkyl benzene sulfonic acid and boric acid are mixed first, a little of water (proper amount) is added for agitation to enable lauryl sodium sulfate, linear alkyl benzene sulfonic acid and boric acid to be fully dissolved, then bromcresol purple aqueous solution is added and is fully and evenly agitated, and finally sodium hydroxide aqueous solution and water are added in the obtained solution.

Description

technical field [0001] The invention relates to a veterinary inspection technology, specifically a diagnostic reagent for detecting subclinical mastitis in mammals such as cattle, sheep and pigs, and is particularly suitable for detecting subclinical mastitis in cows. Background technique [0002] Dairy cow mastitis is one of the common and frequently-occurring diseases of dairy cows. It can be divided into clinical mastitis and recessive mastitis according to whether there are clinical symptoms, most of which are recessive mastitis. Dairy cows suffering from subclinical mastitis have reduced productivity, and their losses account for about 10% of the total annual production capacity; and the total amount of amino acids in milk is reduced by 21%, valine, methionine, isoleucine, leucine, 6 kinds of essential amino acids such as alanine and lysine are significantly reduced; lactose, casein, fat, Ca in milk 2+ ,P 3- 、K + The content of beneficial ingredients such as milk als...

Claims

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Application Information

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IPC IPC(8): G01N33/52G01N21/82G01N21/78
Inventor 罗金印李新圃李宏胜郁杰张礼华王兴亚
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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