Phenolic compound and uses
A technology of compounds and drugs, applied in the field of medicine, can solve the problems of high toxicity and easy generation of drug resistance
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[0065] Take 20 kg of sunflower seeds that are dried and pulverized, use 70% ethanol (150 L) to reflux extract 3 times, each time for 3 hours, and combine the extracts. Ethanol was recovered under reduced pressure at 55°C to obtain 3.0 kg of concentrated extract (LC). Disperse 3.0kg of concentrated extract (LC) with 4.0L of water, and extract three times with equal volumes of petroleum ether, chloroform, ethyl acetate and n-butanol. The extracts were collected and concentrated to obtain 90.21 g of petroleum ether extract (LCP), 91.72 g of chloroform extract (LCC), 55.05 g of ethyl acetate extract (LCE) and 554.35 g of n-butanol extract (LCB).
[0066] Take 55.05 g of ethyl acetate extract (LCE), dissolve it with chloroform and methanol, and weigh 80 g of column chromatography silica gel (100-200 mesh) to mix the sample. Weigh 1200g of silica gel (200-300 mesh), dissolve the silica gel in chloroform and pack the column, and equilibrate the column with chloroform until the silic...
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