Drug sensitivity testing method for bacteria and cells and drug sensitivity testing device

Abstract

The invention discloses a drug sensitivity testing method for bacteria and cells and a drug sensitivity testing device. The testing method comprises the following steps: adding culture solution in a concentrated bacterial specimen or cell specimen to prepare a liquid-state testing specimen; adding coagulant aid into the specimen to ensure that the liquid-state testing specimen is coagulated into a gel shape so as to form a gel testing specimen; pasting drug paper serap to the surface of the gel testing specimen, and putting the specimen in an incubator with the temperature of 37 DEG C for culturing; and observing the drug inhibition zone to determine the sensitivity of the specimen to the drug. The drug sensitivity testing device is a box made of a transparent material, and the coagulant aid is prearranged in the drug sensitivity testing device. The method and the device are characterized in that a colloid raw material is contained in the culture solution, is in liquid state before the coagulant aid is added, and forms stable gel in a certain time at normal temperature after the coagulant aid is added. Therefore, the method and the device sufficiently absorb the advantages of high breeding speed of bacteria or cells in liquid-state culture and simple and convenient drug paper serap testing, and have the characteristics of short testing time, small investment, low application cost and safe and convenient use.

Description

technical field [0001] The invention relates to a drug sensitivity test method and a drug sensitivity test device for bacteria and cells. Background technique [0002] Drug susceptibility testing is an important test for the detection of bacterial or cellular drug resistance. Existing drug susceptibility test method, its used medium is generally divided into two kinds of liquid medium and solid medium, and its test method generally has disc test method and concentration test method, and what disc test method adopted is solid medium , the concentration test method uses a liquid medium. The disc test method is to determine the sensitivity of Mycobacterium tuberculosis to drugs by observing the drug inhibition zone formed by the drug disc attached to the surface of the solid medium; the concentration test method is to detect the concentration of different drugs and different drug concentrations in test containers The growth of bacteria or cells is used to determine the sensit...

AI Technical Summary

Problems solved by technology

The advantages of the existing paper inspection method are easy to operate, simple equipment, and low investment; but it has the following disadvantages: (1) the time required for the entire inspection is too long

If drug therapy is used after the results of drug susceptibility testing are obtained, it will seriously affect the timing of treatment

However, the clinical use of drug treatment without drug susceptibility testing often leads to serious consequences of drug resistance of Mycobacterium tuberculosis

(2) Unsafe

The advantage of this inspection method is that the equipment is simple and the investment is small; but it has the following disadvantages: (1) the required time is long

However, its main disadvantages are that the price of instruments and equipment is high, and the investment is large; the cost of use is high. First, the use cost of instruments and equipment is high. It is difficult for hospitals to bear and popularize

[0004] There are many methods for drug susceptibility testing of in vitro cells. Commonly used methods include MTT colorimetry, radioisotope incorporation, etc., but they all have disadvantages such as troublesome operation and easy to cause wrong test results.

The reason is that it is necessary to prepare multiple test containers with different drugs and different concentrations, and to add cell suspension to each test container. Contamination, which may cause erroneous test results

[0005] As can be seen from the above detailed analysis of tuberculosis drug susceptibility testing, the existing bacteria, cell drug susceptibility testing methods, especially the tubercle bacillus testing method, have the following disadvantages: (1) the required time of the whole testing process is long

(2) troublesome operation

(3) Unsafe

(4) prone to false detection

(5) Large investment, difficult to popularize

Images