Method for determining content of astaxanthin in antarctic krill oil by chromatography

A technology of Antarctic krill oil and astaxanthin, which is applied in the field of chromatographic detection, achieves the effects of good linear relationship, simple method and good reproducibility

Inactive Publication Date: 2010-11-24
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

Currently, there is no literature on the method for the determination of astaxanthin in Antarctic krill oil

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  • Method for determining content of astaxanthin in antarctic krill oil by chromatography
  • Method for determining content of astaxanthin in antarctic krill oil by chromatography
  • Method for determining content of astaxanthin in antarctic krill oil by chromatography

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Embodiment Construction

[0027] The present invention is a method for determining the content of astaxanthin in Antarctic krill oil by gel permeation chromatography purification-high performance liquid chromatography, and the specific peak sequence is all-trans astaxanthin, 9-cis-astaxanthin, 13-cis-Astaxanthin. Astaxanthin esters can be completely converted into free astaxanthin after saponification, and the C30-reversed-phase high-performance liquid chromatography can objectively and truly reflect the content of free astaxanthin.

[0028] A large number of repeated comparative tests were carried out by investigating different test parameters, and finally the best purification conditions, saponification conditions and chromatographic conditions were selected.

[0029] The technical method for determining the content of astaxanthin in Antarctic krill oil of the present invention will be further described in detail below with specific examples and in conjunction with the accompanying drawings.

[0030...

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Abstract

The invention relates to a method for determining the content of astaxanthin in antarctic krill oil by chromatography, which comprises the steps of adopting a gel purification column with a BIO-3X packing, taking ethyl acetate and cyclohexane as eluant according to the proportion of 1:1, and collecting a fractions at 7.48-12.60min; carrying out low-temperature saponification in a solvent formed by methylene dichloride and methanol after purification, and completely converting astaxanthin esters to free astaxanthin; adopting a YMC-Carotenoid C30 chromatographic column; carrying out gradient elution by taking water solution of the methanol, methyl tert-butyl ether and 1% of phosphoric acid as a mobile phase; leading the flow rate to be 1.0mL/min, using an ultraviolet detector as the detector, and leading the detecting wavelength to be 474nm; and determining three isomers of the astaxanthin and adopting the sum of three peak areas for quantifying. The gel chromatographic column can better separate fat from the astaxanthin in the antarctic krill oil, and the adoption of the C30-reversed phase high performance liquid chromatography can accurately determine the content of the astaxanthin, thereby objectively and truly evaluating the quality of the antarctic krill oil.

Description

Technical field: [0001] The present invention relates to a method for chromatographic detection, in particular to a method for determining the content of astaxanthin in Antarctic krill oil by gel permeation chromatography purification-high performance liquid chromatography, that is, a method for purification by gel permeation chromatography, C30 -Reversed-phase high-performance liquid chromatography separation, a method for determining the content of astaxanthin in Antarctic krill oil with an ultraviolet detector. Background technique: [0002] Gel permeation chromatography, as one of the purification means of samples, has been widely used in foreign countries. In my country, the application of GPC in the separation and purification of pesticide residues will become more and more common, especially in fat-rich, In terms of sample separation and purification of macromolecules such as pigments, it has obvious purification effect. [0003] High-performance liquid chromatography...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/74G01N30/60
Inventor 孙伟红冷凯良邢丽红苗钧魁肖荣辉许洋
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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