Method for preparing three-dimensional cell scaffold from elastic microsphere pore-forming agent

Abstract

The invention discloses a method for preparing a three-dimensional cell scaffold from an elastic microsphere pore-forming agent. The method is characterized by comprising the following steps of: 1) preparing the elastic microsphere pore-forming agent and preparing the solution of a scaffold material; 2) placing the elastic microsphere pore-forming agent into a mould, adding the solution of the scaffold material to immerse the pore-forming agent, uniformly mixing the elastic microsphere pore-forming agent and the solution of the scaffold material to obtain a scaffold / elastic microsphere pore-forming agent complex, filter-pressing the complex by using a filter press plate with distributed microspheres to uniformly distribute the elastic microsphere pore-forming agent in the solution and contact with each other, and pre-freezing the complex for 3 hours at the temperature of -10 DEG C or lower; 3) demolding the complex and freeze-drying the complex for 6 to 8 hours; 4) rehydrating the complex in distilled water for 1 hour; and 5) removing the pore-forming agent. The scaffold preparation method of the invention can realize manual controllability of the pore structure of the scaffold and the preparation process is simple, convenient and easy.

Description

technical field [0001] The invention belongs to the field of biomaterials, and in particular relates to a method for preparing a three-dimensional cell scaffold by combining an elastic microsphere porogen with a filter press forming technology. Background technique [0002] In tissue engineering, cells rely on scaffolds as growth templates. Cell scaffolds not only guide tissue regeneration, but also control the properties of tissues or organs. Therefore, three-dimensional porous scaffolds play a key role in tissue engineering and are one of the important fields of tissue engineering research. . A large number of studies have shown that the pore shape, size, and porosity of the cell scaffold directly affect the migration, differentiation, proliferation, and tissue formation of the planted cells. An ideal cell scaffold must have a certain range of pores and pore communication channels. It has been reported that the main factors affecting the growth of bone tissue into the por...

AI Technical Summary

Problems solved by technology

Among the various traditional technologies for preparing cell scaffolds, the fiber bonding method can obtain scaffolds with good connectivity between pores, but the pore structure is difficult to control; although gas foaming and phase separation techniques can prepare large-volume porous scaffolds, the pores There is no guarantee of complete connectivity between the scaffolds, and the size of the pores and the size of the channels between the pores cannot be controlled; the scaffolds prepared by the solvent casting / particle leaching method have poor connectivity and rough pore morphology.

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