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Enterovirus and application thereof

A technology for enterovirus and hand, foot and mouth disease, applied in antiviral agents, viruses/bacteriophages, medical preparations containing active ingredients, etc., can solve problems such as health hazards for infants and young children

Inactive Publication Date: 2012-10-10
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the disease has been reported before, it mostly appeared as sporadic cases. In 2009, it broke out in many areas of Anhui, Henan and Hebei in my country, causing serious harm to the health of infants and young children.

Method used

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  • Enterovirus and application thereof
  • Enterovirus and application thereof
  • Enterovirus and application thereof

Examples

Experimental program
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Embodiment 1

[0013] Example 1. Obtainment of Enterovirus 71 Henan isolate 2

[0014] 1. Isolation of Enterovirus 71 (Enterovirus 71) Henan isolate 2

[0015] The inventor of the present invention collects a serum sample of a patient with hand, foot and mouth disease that has been clinically identified, and then performs virus isolation. The virus isolation method samples are directly inoculated into Vero cells. When the cytopathic effect reaches 95%, the virus culture supernatant is collected. After the round of plaques were purified, single plaques were picked and infected with Vero cells for virus proliferation. The cell suspension was collected to obtain the virus liquid. Finally, the inventors isolated an enterovirus EV71 type virus from the samples collected in Henan Province. This enterovirus type EV71 was named Enterovirus type EV71 (Enterovirus 71) Henan isolate 2. Subsequently, studies on virus characteristics and pathogenicity were carried out.

[0016] Enterovirus 71 (Enterovirus 71)...

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Abstract

The invention discloses an enterovirus and application thereof. The enterovirus has a name: Henan isolates 2 of an EV (Enterovirus) 71 type and has a preservation registration number CGMCC No. 3675. The enterovirus has stronger pathogenicity and can be used for establishing an animal model of hand, foot and mouth diseases and developing vaccines.

Description

Technical field [0001] The invention relates to an enterovirus and its application. Background technique [0002] Hand-Foot-mouth disease (HFMD) is a common infectious disease caused by a variety of enteroviruses. In recent years, it has occurred in tropical regions of the Asia-Pacific region, mainly in infants and young children. Enteroviruses that cause hand, foot and mouth disease include enterovirus type EV71 (Enterovirus 71, or EV-71 for short) and group A Coxsackie virus (CoxA), Echo virus (Echo) and so on. Among them, EV-71 is the main pathogen causing hand, foot and mouth disease, often leading to severe neurological syndrome. EV-71 is a single-stranded positive-stranded RNA virus, divided into A, B, C3 genotypes, B1-B4 and C1-C4 total 8 subtypes. [0003] Although the disease has been reported before, it mostly appeared in sporadic cases. In 2009, it broke out in Anhui, Henan and Hebei in my country, causing serious harm to the health of infants and young children. Ther...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A01K67/027A61K39/125A61P31/14
Inventor 常国辉吴晓燕林磊罗彦军祝庆余
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI