Super-resolution fluorescence microscopy method and device based on tangential polarization
A super-resolution fluorescence and tangential polarization technology, applied in the field of fluorescence microscopy, can solve the problems of difficult focusing of circularly polarized excitation light, reduced imaging resolution and imaging quality, etc. Effect
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[0050] The present invention will be described in detail below with reference to the drawings and embodiments, but the present invention is not limited to this.
[0051] Such as figure 1 The shown super-resolution fluorescence microscopy device based on tangential polarization includes:
[0052] The first laser 1, the second laser 2, the first tangential polarization converter 3, the second tangential polarization converter 6, the vortex-distributed 0~2π phase plate 4, the first dichroic mirror 5, the second dichroic Mirror 7, high numerical aperture achromatic lens 8, nano-translation stage 10, narrow band filter 11, achromatic focusing lens 12, pinhole 13, and detector 14.
[0053] The first tangential polarization converter 3 and the second tangential polarization converter 6 are the Radial-Azimuthal Polarization Converter of Sweden (ARCoptix), and the 0~2π phase plate 4 of the vortex distribution is the vortex of RPC photonics of the United States. Rotation phase plate (Vortex 0...
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