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Extraction technology of total alkaloids of sophora alopecuroides

An extraction process, the technology of Sophora sophora, which is applied in the field of extraction process of alkaloids from Sophora sophora, can solve the problems of large dosage of solid preparations, poor clarity of injections, and large consumption of chitosan, etc., to achieve improved clarification process, Ease of preservation and saving of production cost

Inactive Publication Date: 2010-12-15
北京九草堂药物研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent CN1110339C discloses "Preparation Technology of Sophora Sophorae Total Alkali", the process is complicated, and the total alkali yield of Sophora Sophora Sophora is less than 3%. According to a large number of research results at home and abroad, it is shown that the aerial part of Sophora Sophora Sophora contains about 6.11% of total alkali. 8.03%, seeds contain about 8.11%
[0005] The water extract of traditional Chinese medicine often contains impurities such as starch, polysaccharide, protein, mucus, tannin, pigment, resin, pectin, etc., so that the dosage of the prepared solid preparation is large, and the clarity of the liquid preparation, such as oral liquid and injection, is not good.
In order to overcome this disadvantage, in the pharmaceutical process, chitosan is often used as a clarifying agent, but in large-scale production, it often occurs that the chitosan solution directly prepared has a colloidal solution with an incomplete solvent, resulting in poor clarification effect, Problems such as large consumption of chitosan

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) crushing: crushing the seeds of Sophora Sophora to coarse powder;

[0032] (2) Warm leaching: pre-soak the coarse powder with 40% ethanol with a pH value of 2 for 30 minutes, then leaching at 50°C for 12 hours, with a solid-to-liquid ratio of 1:8, and leaching hydraulic filtration to obtain the filtrate;

[0033] (3) clarification: add the improved chitosan in the filtrate, stir, leave standstill, get the supernatant;

[0034] (4) concentrating: concentrating the supernatant obtained in step (3), reclaiming ethanol to obtain a concentrated solution;

[0035] (5) Alkalinization extraction: adjust the pH value of the concentrated solution to 10, and extract with n-butanol (50%, v / v) to obtain an n-butanol layer and an alkaline water layer;

[0036] (6) Acid water shift extraction: take the n-butanol layer gained in step (5), and use 2% acid aqueous solution for shift extraction until there is no alkaloid reaction in the n-butanol, and the resulting acid water liquid ...

Embodiment 2

[0040] (1) Pulverization: the root of Sophora Sophora is pulverized into coarse powder;

[0041] (2) Warm immersion: the coarse powder obtained in step (1) is pre-soaked with 50% ethanol with a pH value of 3 for 50 minutes, and then leached for 15 hours at 50° C., with a solid-to-liquid ratio of 1:10. Hydraulic filtration to obtain filtrate;

[0042] (3) clarification: add the improved chitosan in the filtrate, stir, leave standstill, get the supernatant;

[0043] (4) concentrating: concentrating the supernatant obtained in step (3), reclaiming ethanol to obtain a concentrated solution;

[0044] (5) Alkalinization extraction: adjust the pH value of the concentrated solution to 11, and extract with n-butanol (50%, v / v) to obtain an n-butanol layer and an alkaline water layer;

[0045] (6) Acid water shift extraction: take the n-butanol layer gained in step (5), and use 2% acid aqueous solution for shift extraction until there is no alkaloid reaction in the n-butanol, and the ...

Embodiment 3

[0049] (1) Pulverizing: the stem and leaf of Sophora Sophora are pulverized into coarse powder;

[0050] (2) Warm soaking: the coarse powder obtained in step (1) is pre-soaked with 30% ethanol with a pH value of 3 for 50 minutes, and then leached for 12 hours at 60° C., with a solid-to-liquid ratio of 1:12. Hydraulic filtration to obtain filtrate;

[0051] (3) clarification: add the improved chitosan in the filtrate, stir, leave standstill, get the supernatant;

[0052] (4) concentrating: concentrating the supernatant obtained in step (3), reclaiming ethanol to obtain a concentrated solution;

[0053] (5) Alkalinization extraction: adjust the pH value of the concentrated solution to 12, and extract with n-butanol (50%, v / v) to obtain an n-butanol layer and an alkaline water layer;

[0054] (6) Acid water shift extraction: take the n-butanol layer gained in step (5), and use 2% acid aqueous solution for shift extraction until there is no alkaloid reaction in the n-butanol, an...

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PUM

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Abstract

The invention provides an extraction technology of total alkaloids of sophora alopecuroides, overcoming the defects of complex technology, low yield and big toxicity of adopted solvent in the prior art. The technologies of smashing, extracting at low temperature, clarifying, extracting in an alkalization mode, extracting in an acidification shifting mode, drying by microwave in vacuum and the like are adopted to obtain the powder of dry sophora alopecuroides. The total alkali yield is 5.7%, and the invention is suitable for industrial big production.

Description

technical field [0001] The invention relates to an extraction process of alkaloids in Sophora sophorae, in particular to an extraction process of total alkaloids from Sophora sophora. Background technique [0002] Sophora alopecuraides L. is a plant of the genus Sophora in the family Fabaceae, also known as bitter licorice, bitter bean root, western bean root, and sophora flavescens. It is widely distributed in Northwest my country. The output in China accounts for 62% of the world's output. The resources are very rich. [0003] The total alkaloids of Sophora Sophora is a general term for the total extract of Sophora Sophora, which contains most of the alkaloids contained in Sophora Sophora. Sophora alopecia alkaloids are widely used clinically for their good drug activity. They not only have anti-cancer, tumor suppressor, inhibition and killing various microorganisms, but also have a wide range of pharmacological effects on the immune system, nervous system and cardiovascul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/489A61P31/00A61P9/00A61P25/00A61P35/00A61P37/02A61K125/00A61K127/00A61K131/00A61K135/00
Inventor 雷思琦许冠英白景玲陈丽平
Owner 北京九草堂药物研究院有限公司
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