1,4-cyclohexane diformyl based preparation method of transparent hydrogel
A technology of cyclohexanediformyl and hydrogel, which is applied in chemical instruments and methods, colloid chemistry, colloid chemistry, etc., can solve the problems of harsh synthesis, opacity, and limitation, and achieve easy operation and good water absorption , the effect of high yield
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[0037] Example one
[0038] Preparation of transparent hydrogel 1
[0039]
[0040] 1. Dissolve 25 mg of the above gel molecules with 40 μl (1.9M) hydrochloric acid, and dilute to 250 μl with ultrapure water to obtain a 10% solution 1. Then, 225 mg of sucrose was dissolved in the above solution to obtain a mixed solution containing 45% sucrose.
[0041] 2. Take 50μl of solution 1 and place it in a 2ml transparent tube or bottle.
[0042] 3. Then add 450μl DMEM dropwise to the above tube or bottle and shake it by hand. Finally, a mixed solution of organic molecules containing 1% of 1,4-cyclohexanediformyl and 9% of sucrose is obtained.
[0043] 4. Leave it to stand, the solution will begin to form a gel after 5 minutes, and the overall solution can form a hard gel after 30 minutes.
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[0044] Example two
[0045] Preparation of transparent hydrogel 2
[0046]
[0047] 1. Dissolve 25 mg of the above gel molecules with 40 μl (1.9M) hydrochloric acid, and dilute to 250 μl with ultrapure water to obtain a 10% solution 1.
[0048] 2. Take 450 μl of DMEM tissue culture solution and dissolve 45 mg of sucrose in the above solution to obtain a solution containing 10% sucrose.
[0049] 3. Take 50μl of solution 1 and place it in a 2ml transparent tube or bottle. Then, add 450 μl of DMEM solution containing sucrose dropwise to the above-mentioned tube or bottle and shake it by hand. Finally, a mixed solution of organic molecules containing 1% of 1,4-cyclohexanediformyl and 9% of sucrose is obtained.
[0050] 4. Leave it to stand, the solution will begin to form a gel after 3 minutes, and the whole solution can form a hard gel after 30 minutes.
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