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Method for identifying Nigella glandulifera Freyn medicinal material

A technology of Nigella cumulus and medicinal materials, which is applied in the field of identification of traditional Chinese medicinal materials, and achieves the effects of good stability and repeatability, high precision, and simple method

Inactive Publication Date: 2010-12-29
XINJIANG INST OF MATERIA MEDICA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a method for identifying the medicinal material of Nigella tumefica, which overcomes the deficiencies in the prior art, and establishes the method for the identification of Nigella tumefaction by high performance liquid chromatography. The standard fingerprint method achieves the identification of the medicinal material of Nigella sativa, thus solving the problem of quality control of the medicinal material of Nigella spp. Authenticity identification and quality control of Nigella sativa L.

Method used

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  • Method for identifying Nigella glandulifera Freyn medicinal material
  • Method for identifying Nigella glandulifera Freyn medicinal material
  • Method for identifying Nigella glandulifera Freyn medicinal material

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Embodiment Construction

[0026] In order to better understand the present invention, the present invention will be further described below in conjunction with the examples, but the present invention is not limited thereto.

[0027] Embodiment of the present invention: identification method of the medicinal material of Nigella sativa.

[0028] 1 Instruments and reagents.

[0029] 1.1 Instruments.

[0030] Shimadzu LC-10ADvp high performance liquid chromatograph, SPD-M10Avp detector.

[0031] 1.2 Test drug.

[0032] A total of 10 batches of Nigella officinalis medicinal materials were collected from Xinjiang Hotan, Hotan Yutian, Kashgar, Kashgar Yuepu Lake, Qitai, Turpan, Yili, Shanshan and other places; the nigella glycoside reference substance was self-made, with a purity of more than 98 %; Acetonitrile is chromatographically pure, and other reagents are analytically pure.

[0033] 2 Methods and results.

[0034] 2.1 Determination of chromatographic conditions.

[0035] 2.2.1...

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Abstract

The invention relates to the technical field of Chinese medicinal material indentifying methods, in particular to a method for identifying a Nigella glandulifera Freyn medicinal material, which comprises the following steps of: 1, establishing a standard fingerprint of the Nigella glandulifera Freyn medicinal material; 2, testing a fingerprint of a sample of the Nigella glandulifera Freyn medicinal material to be tested by the step same as the step 1; and 3, comparing the fingerprint of the sample of the Nigella glandulifera Freyn medicinal material to be tested with the standard fingerprint of the Nigella glandulifera Freyn medicinal material, and screening the Nigella glandulifera Freyn medicinal material according with the standard fingerprint of the Nigella glandulifera Freyn medicinal material. Compared with the prior art, the method has the characteristics of simpleness, convenience, high accuracy, and high stability and repeatability. The standard fingerprint established by the method is utilized to effectively identify the Nigella glandulifera Freyn medicinal material and effectively monitor and control the inner quality of the Nigella glandulifera Freyn medicinal material.

Description

technical field [0001] The invention relates to the technical field of identification methods for Chinese medicinal materials, and relates to an identification method for the medicinal material Nigella tumefaction, in particular to a method for identifying the medicinal material Nigella tumefaction by using high-performance liquid chromatography fingerprints. Background technique [0002] Nigella sativa is a traditional medicinal material of the Uyghur nationality, and it is the dry mature seed of Nigella gram landulifera Freyn, a plant of the genus Nigella (Nickella) in the family Ranunculaceae (Raunnculaceae). Nigella nigella is also known as nigella glandularis, and its Uighur name is Si Yadan. It is mainly produced in Xinjiang and Xishuangbanna, Yunnan. It is planted in most areas of Xinjiang, such as Turpan, Shanshan and other places, where resources are abundant; It can be bought in most medicinal materials markets in Xinjiang and has a wide range of applications. Bla...

Claims

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Application Information

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IPC IPC(8): A61K36/71G01N30/02G01N30/06G01N30/36A61P11/00A61P11/14A61P11/06A61P25/00A61P7/10A61P27/16A61P15/00A61P15/14A61P13/04A61P17/14A61P25/20A61P29/00A61P19/02A61P21/00A61P19/00A61P25/08A61P1/08A61P7/06A61P1/00A61P1/14A61P1/12A61P1/16
Inventor 徐芳赵军徐建国杨伟俊韩立新刘亚婷谭为陈燕
Owner XINJIANG INST OF MATERIA MEDICA
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