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Turtle shell immunoadjuvant and influenza vaccine containing the same

A technology of immune adjuvant and influenza vaccine, applied in the direction of medical preparations containing active ingredients, antiviral agents, antibody medical components, etc., can solve the problems of single immune adjuvant, large demand for vaccines, and unavoidable side effects, etc., to achieve The effect of simple preparation method

Active Publication Date: 2010-12-29
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] In order to solve the current problems of single immune adjuvant, unavoidable side effects, limited supply of vaccine production, difficulty in coping with the large demand for vaccines when infectious diseases break out, and slow onset time of vaccines, the invention provides a vaccine that can improve the immune effect of vaccines and at the same time Turtle shell immune adjuvant that brings huge economic and social benefits

Method used

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  • Turtle shell immunoadjuvant and influenza vaccine containing the same
  • Turtle shell immunoadjuvant and influenza vaccine containing the same
  • Turtle shell immunoadjuvant and influenza vaccine containing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The preparation of the soft-shelled turtle shell ethanol extract: get dry turtle shell 40g, pulverize into powder, be placed in the container, be 65% ethanol with 4 times that is 160ml mass concentration, carry out heating and reflux extraction as usual, wherein, each heating temperature is 50°C, the reflux time is 60 minutes, and the number of extractions is 2 times. After filtration, the filtrate is decompressed to recover ethanol, and the remaining filtrate is concentrated and dried to obtain an extract.

Embodiment 2

[0049] The preparation of water extract of soft-shelled turtle shell: take 40g of dried soft-shelled turtle shell, pulverize it into powder, put it in a container, heat and decoct it with 8 times of 320ml distilled water as usual, wherein, each decoction time is 4 hours, and the extraction times for 3 times, filtered, and after combining the filtrates, the filtrates were concentrated and dried to obtain extracts.

Embodiment 3

[0051] Preparation of nanoemulsion vehicle:

[0052] 1) Prepare materials according to the following mass ratio: 2g polyoxyethylene castor oil, 2g polyethylene glycol 400, 2g isopropyl myristate, 1.07g water for injection;

[0053] 2) Add 2g of polyoxyethylene castor oil and 2g of polyethylene glycol 400 into the mixing and stirring bottle, and keep stirring for 5 minutes at a speed of 300 rpm at room temperature, and then add 2g of isopropyl myristate after mixing , mixed uniformly to obtain a mixture;

[0054] 3) 1.07 g of water for injection was dropped into the mixture of the above 2), and at the same time, at a rotating speed of 300 rpm, at room temperature, the mixture was stirred until the liquid was transparent to obtain a nanoemulsion carrier;

[0055] 4) The nanoemulsion carrier prepared in 3) is detected as follows: 1. through Malvern particle size detector detection, the particle size range is 28.706~46.952nm, qualified; 2. through 3000rpm, 15min centrifugation, t...

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Abstract

The invention relates to a turtle shell immunoadjuvant and an influenza vaccine containing the immunoadjuvant; the turtle shell immunoadjuvant comprises the following ingredients in percentage by weight: 0.001-100% of turtle shell extractive and 0-99.999% of carriers. The composition of the influenza vaccine containing the turtle shell immunoadjuvant is as follows: the mass ratio of the turtle shell immunoadjuvant to the influenza vaccine hemagglutinin is 50-180050. The turtle shell immunoadjuvant in the invention has a good immunoadjuvant effect and has an effect of effectively preventing diseases in human and animal vaccine. The turtle shell extractive is used as the immunoadjuvant of the influenza vaccine; compared with the traditional aluminum hydroxide immunoadjuvant, the turtle shell immunoadjuvant has high using safety and small byeffect, can well improve the original nature of the influenza vaccine; in the process of preparing the influenza vaccine, the method is simple, economic and environment friendly and convenient; the method easily controls the mass, greatly reduces the dosage of the influenza vaccine antigen and satisfies the immunity demand when occurring the epidemic influenza.

Description

technical field [0001] The invention relates to a turtle shell immune adjuvant, and also relates to an influenza vaccine containing the turtle shell immune adjuvant, which belongs to the field of biotechnology. technical background [0002] Turtle shell is the carapace of soft-shelled turtle (Trionyx sinensis Wiegmann), which is mainly produced in Hubei, Hunan, Anhui and other places. Yes, they can be caught all year round, mostly in autumn and winter. After killing, scald them in boiling water until the hard skin on the carapace can peel off, remove the residual meat, dry them in the sun, or fry them with sand and then quench them with vinegar. The turtle shell was first recorded in "Shen Nong's Materia Medica", and it has been recorded in successive dynasties of herbal medicines. Salty in nature, slightly cold, returns to Liver and Kidney channels. Shu version "On the Nature of Medicine" contains "curing fatigue and removing bone fever". "Compendium of Materia Medica" c...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/145A61P31/16
Inventor 李映波程永现陈芹芹吕青李慧
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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