Serum calcium standard substance

A standard substance, serum calcium technology, applied in the field of serum calcium standard substance, can solve the problems of high price and long purchase cycle

Active Publication Date: 2010-12-29
BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Foreign standard materials are expensive, and the purchase cycle is longer due to consignment and other factors

Method used

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  • Serum calcium standard substance
  • Serum calcium standard substance
  • Serum calcium standard substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Embodiment 1, preparation and effect detection of serum calcium standard substance I

[0070] 1. Collection of serum

[0071] Serum samples were collected from the laboratory department of Beijing Chaoyang Hospital. The remaining serum after physical examination was collected. The age ranged from 20 to 70 years. All serum samples were collected with the consent of the subjects. The serum collection method followed ISO Guide 34 (ISO Guide 34, General requirements for the competence of reference material producers.).

[0072] Collect the serum in a 50ml polyethylene airtight centrifuge tube, sterilize the surface of the bottle with 75% alcohol and seal it. The appearance is clear and transparent, except for abnormal traits such as hemolysis and chyle. A total of 5,000 individual serums were collected without any additives or preservatives, mixed, and filtered to obtain a total of about 3,000 ml of mixed serum, which was stored in a -80°C refrigerator for later use.

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Embodiment 2

Embodiment 3

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PUM

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Abstract

The invention discloses a candidate serum calcium standard substance. A method for preparing the candidate serum calcium standard substance comprises the following steps of: diluting in-vitro serum or adding calcium into the in-vitro serum to obtain 5 kinds of serum solution with different calcium concentrations; and fixing a value for each of the 5 kinds of serum solution with different calcium concentrations respectively so as to obtain serum calcium standard substances with 5 calcium concentrations. In the candidate serum calcium standard substance, other substance is not added and only the serum is used as a substrate; the candidate serum calcium standard substance has the advantages of high interoperability, high uniformity, high stability under the -80 DEG C storage condition, reliable traceability of the fixed value and relatively small relative synthesis uncertainty of between 0.83 and 1.55 percent; moreover, compared with other conventional serum calcium standard substances, the candidate serum calcium standard substance has the advantages of low cost and simple preparation method.

Description

technical field [0001] The invention relates to a serum calcium standard substance. Background technique [0002] Calcium in the human body mainly includes intracellular calcium and extracellular calcium. Intracellular calcium mainly exists in mitochondria, sarcoplasm and endoplasmic reticulum, which is responsible for triggering muscle excitation-contraction coupling and participating in various intracellular signal transduction as a second messenger. Lead, participate in the regulation of self and phosphorus metabolism and as cofactor of enzyme. Extracellular calcium mainly exists in plasma, which can affect the stress of nerve cell membrane and participate in the process of blood coagulation. Blood calcium is about 2.5mmol / L. It exists in serum in two forms: diffuse calcium and non-diffusible calcium. Non-diffusible calcium accounts for about 45% of the total serum calcium. It refers to calcium bound to serum protein and cannot penetrate capillaries. wall, and binding i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/96G01N21/31
Inventor 王清涛马怀安李惠玲王金英徐静贾慧敏童清
Owner BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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