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Method for preparing nanoliposomes of medium chain fatty acids (MCFAs) by dynamic high-pressure microfluidization (DHPM)-freezing and thawing method

A high-pressure micro-jet, medium-chain fatty acid technology, applied in the field of food nutrition, can solve the problems of large toxic reagents, unsuitable for industrial production, etc., and achieve the effect of stable liposome properties and good storage stability

Inactive Publication Date: 2012-06-20
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation process of these traditional methods is simple, but often introduces a large amount of toxic reagents, and is not suitable for large-scale industrial production

Method used

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  • Method for preparing nanoliposomes of medium chain fatty acids (MCFAs) by dynamic high-pressure microfluidization (DHPM)-freezing and thawing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Weigh 0.45g of medium-chain fatty acids (MCFAs), 2.06g of lecithin, 0.34g of cholesterol, 0.62g of Tween-80 and 0.04g of vitamin E, dissolve them completely in 42ml of absolute ethanol, and remove them by vacuum rotation in a water bath at 40°C. Anhydrous ethanol, forming a uniform film. Take 30ml of phosphate buffered saline solution (PBS) with a concentration of 0.05M and a pH of 7.4, add 0.9g of maltose, add the obtained maltose solution to the film for hydration and wash the film, and the uniform suspension formed is the thick liposome. The crude liposomes were added to DHPM, and subjected to microfluidization treatment 4 times under the condition of 140MPa to obtain medium-chain fatty acid (MCFAs) liposomes. Put medium-chain fatty acid (MCFAs) liposomes in -80°C for 0.5 hours, take them out, thaw them in a water bath at 40°C for 15 minutes, and freeze them again in -80°C. Repeat this process 3 times to obtain medium-chain fatty acids (MCFAs) Nanoliposomes. The pr...

Embodiment 2

[0020] Weigh 0.90g of medium-chain fatty acids (MCFAs), 4.12g of lecithin, 0.68g of cholesterol, 1.23g of Tween-80 and 0.08g of vitamin E, dissolve them completely in 83ml of absolute ethanol, and remove them by vacuum rotation in a water bath at 40°C. Anhydrous ethanol, forming a uniform film. Take 60ml of phosphate buffered saline solution (PBS) with a concentration of 0.05M and a pH of 7.4, add 1.8g of maltose, add the resulting maltose solution to the film for hydration and wash the film, and form a uniform suspension that is thick liposomes. The crude liposomes were added to DHPM, and subjected to microfluidization treatment 4 times under the condition of 140MPa to obtain medium-chain fatty acid (MCFAs) liposomes. Put medium-chain fatty acid (MCFAs) liposomes in -80°C for 0.5 hours, take them out, thaw them in a water bath at 40°C for 15 minutes, and freeze them again in -80°C. Repeat this process 3 times to obtain medium-chain fatty acids (MCFAs) Nanoliposomes. The pre...

Embodiment 3

[0022] Weigh 0.45g of medium-chain fatty acids (MCFAs), 2.06g of lecithin, 0.34g of cholesterol, 0.84g of Tween-80 and 0.08g of vitamin E, dissolve them completely in 42ml of absolute ethanol, and remove them by vacuum rotation in a water bath at 40°C. Anhydrous ethanol, forming a uniform film. Take 30ml of phosphate buffered saline solution (PBS) with a concentration of 0.05M and a pH of 7.4, add 0.9g of maltose, add the obtained maltose solution to the film for hydration and wash the film, and the uniform suspension formed is the thick liposome. The crude liposomes were added to DHPM, and subjected to microfluidization treatment 4 times under the condition of 140MPa to obtain medium-chain fatty acid (MCFAs) liposomes. Put medium-chain fatty acid (MCFAs) liposomes in -80°C for 1.0h, take them out, thaw them in a water bath at 40°C for 15 minutes, put them in -80°C for freezing again, and repeat this process 3 times to obtain medium-chain fatty acids (MCFAs) Nanoliposomes. T...

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Abstract

The invention relates to a preparation method of nanoliposomes of medium chain fatty acids (MCFAs), which is used for preparing nanoliposomes of MCFAs by taking liposoluble MCFAs as raw materials and lecithin and cholesterol as wall materials and adopting a dynamic high-pressure microfluidization (DHPM)-freezing and thawing method through the processes of dissolving, uniformly mixing, removing solvents in vacuum, washing a membrane by hydration, treating by DHPM, repeatedly freezing and thawing and the like. The nanoliposomes of the MCFAs, which are prepared by the invention, have the characteristics that the envelopment rate is 40%-50%, the mean particle size is 90-110nm, the dispersion coefficient is less than 0.10, and the zeta potential is (-30mV)-(-50mV); and the nanoliposomes have the advantages of uniform distribution, high bioavailability and good storage stability.

Description

technical field [0001] The invention relates to the field of food nutrition, in particular to a method for preparing medium-chain fatty acid nanoliposomes. Background technique [0002] From the perspective of nutritional physiology, medium-chain fatty acids (MCFAs) refer to octanoic acid (C8:0) with 8 carbon elements and decanoic acid (C10:0) with 10 carbon elements. . Compared with long-chain fatty acids (LCFAs) absorbed in the lymphatic system, medium-chain fatty acids (MCFAs) have many unique physiological functions such as fast absorption, difficult accumulation in the body, and easy energy supply. It is: (1) medium-chain fatty acids (MCFAs) have low melting point, small molecular weight, liquid state at room temperature, and low energy value; (2) do not rely on L-carnitine and directly pass through the mitochondrial double membrane and enter the mitochondria for oxidation , to quickly provide energy for the body, and can be used as an energy source for athletes, pati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/302A23P1/00A23L33/15A23P10/00
Inventor 刘成梅刘伟刘玮琳郑会娟梁瑞红王瑞莲
Owner NANCHANG UNIV