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Preparation of medium chain fatty acid nanoliposome by using film dispersion-dynamic high-pressure microjet

A medium-chain fatty acid and high-pressure micro-jet technology, which is applied in the field of food nutrition, can solve the problems of unsuitable large-scale industrial production, use, and large toxic reagents, and achieve good stability, high bioavailability, and low leakage rate.

Inactive Publication Date: 2012-08-29
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the preparation process of these methods is simple, the main disadvantage is that a large amount of toxic reagents are used, and it is not suitable for large-scale industrial production.

Method used

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  • Preparation of medium chain fatty acid nanoliposome by using film dispersion-dynamic high-pressure microjet

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Weigh 0.45g of medium-chain fatty acids (MCFAs), 2.06g of lecithin, 0.34g of cholesterol, 0.62g of Tween-80 and 0.04g of vitamin E, dissolve them completely in 42ml of absolute ethanol, and remove them by vacuum rotation in a water bath at 40°C. Anhydrous ethanol, forming a uniform film. Add 30ml of phosphate buffered saline (PBS) with pH7.4 and a concentration of 0.05M to wash the membrane, and the uniform suspension formed is the thick liposome. Add the crude liposomes into DHPM, and microfluidize them four times under the condition of 140MPa to prepare medium-chain fatty acid (MCFAs) nanoliposomes. The prepared medium-chain fatty acid (MCFAs) nano-liposome is a transparent light yellow solution with an encapsulation efficiency of 73.33%, an average particle diameter of 70.5nm, a dispersion coefficient of 0.187 and a zeta potential of -44.07mV.

Embodiment 2

[0019] Weigh 0.90g of medium-chain fatty acids (MCFAs), 4.12g of lecithin, 0.68g of cholesterol, 1.20g of Tween-80 and 0.08g of vitamin E, dissolve them completely in 83ml of absolute ethanol, and remove them by vacuum rotation in a water bath at 40°C. Anhydrous ethanol, forming a uniform film. Add 60ml of phosphate buffered saline (PBS) with pH7.4 and a concentration of 0.05M to wash the membrane, and the uniform suspension formed is the thick liposome. Add the crude liposomes into DHPM, and microfluidize them four times under the condition of 140MPa to prepare medium-chain fatty acid (MCFAs) nanoliposomes. The prepared medium-chain fatty acid (MCFAs) nano-liposome is a transparent light yellow solution with an encapsulation efficiency of 70.48%, an average particle diameter of 78.9nm, a dispersion coefficient of 0.191 and a zeta potential of -38.93mV.

Embodiment 3

[0021] Weigh 0.45g of medium-chain fatty acids (MCFAs), 2.06g of lecithin, 0.34g of cholesterol, 0.82g of Tween-80 and 0.08g of vitamin E, dissolve them completely in 42ml of absolute ethanol, and remove them by vacuum rotation in a water bath at 40°C. Anhydrous ethanol, forming a uniform film. Add 30ml of phosphate buffered saline (PBS) with pH7.4 and a concentration of 0.05M to wash the membrane, and the uniform suspension formed is the thick liposome. Add the crude liposomes into DHPM, and microfluidize them four times under the condition of 140MPa to prepare medium-chain fatty acid (MCFAs) nanoliposomes. The prepared medium-chain fatty acid (MCFAs) nano-liposome is a transparent light yellow solution with an encapsulation efficiency of 72.68%, an average particle diameter of 84.1nm, a dispersion coefficient of 0.180 and a zeta potential of -45.44mV.

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Abstract

The invention relates to a preparation method of medium chain fatty acid (MCFAs) nanoliposome which is prepared by using fat-soluble MCFAs as a raw material and lecithin and cholesterin as wall materials and adopting a film dispersion-dynamic high-pressure microjet (DHPM) method as well as comprising the steps of dissolving, evenly mixing, desolventizing in vacuum, washing a film in a hydration mode and processing the DHPM. In the prepared nanoliposome, an encapsulation rate reaches above 70 percent, the average granularity is 70-100nm, a dispersion coefficient is smaller than 0.20, and the zeta potential is (-30mV)-(-50mV). The prepared nanoliposome has the advantages of even distribution, low leakage rate and good long-period storage stability.

Description

technical field [0001] The invention relates to the field of food nutrition, in particular to a method for preparing medium-chain fatty acid nanoliposomes. Background technique [0002] From the perspective of nutritional physiology, medium-chain fatty acids (MCFAs) refer to octanoic acid (C8:0) with 8 carbon elements and decanoic acid (C10:0) with 10 carbon elements. . Medium-chain fatty acids (MCFAs) have many unique physiological functions. Compared with long-chain fatty acids (LCFAs) absorbed in the lymphatic system, they have fast absorption, are not easy to accumulate in the body, and are easy to provide energy. The characteristics are mainly manifested in the following aspects: (1) medium-chain fatty acids (MCFAs) have low melting point, small molecular weight, liquid state at room temperature, and low energy value; (2) do not rely on L-carnitine as a carrier, and directly pass through the mitochondria Double-layer membrane enters the mitochondria, oxidizes rapidly,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/127A23L1/302A23P1/00A23L33/15A23P20/18
Inventor 刘伟刘玮琳刘成梅郑会娟梁瑞红王瑞莲
Owner NANCHANG UNIV