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Bacteria YXY-C1 for preparing gamma-polyglutamic acid by solid fermentation and products thereof

A technology of YXY-C1 and polyglutamic acid, which is applied in the direction of fermentation, bacteria, and microorganism-based methods, can solve the problems of polluting the environment, wasting nutrient resources, etc., and achieves low energy consumption, simple process, simple and efficient product recovery Effect

Inactive Publication Date: 2011-01-19
江苏谷荣茂生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] On the other hand, the waste of livestock and poultry manure after large-scale breeding and the leftovers of the food fermentation industry are not only seriously polluted the environment, but also greatly waste a large amount of nutrient resources (C, N, P, K, S and trace elements )

Method used

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  • Bacteria YXY-C1 for preparing gamma-polyglutamic acid by solid fermentation and products thereof
  • Bacteria YXY-C1 for preparing gamma-polyglutamic acid by solid fermentation and products thereof
  • Bacteria YXY-C1 for preparing gamma-polyglutamic acid by solid fermentation and products thereof

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Embodiment Construction

[0032] (1) Strain isolation and identification

[0033] Medium:

[0034] Solid selective medium (g / L): citric acid 10, glutamic acid 10, NH 4 C16,K 2 HPO 4 1. MgSO 4 ·7H 2 O 0.5, FeCl 3 ·6H 2 O 0.02, CaCl 2 0.2, MnSO 4 ·H 2 O 0.05, agar 20, pH 7.2.

[0035] Seed liquid medium (g / L): peptone 10, yeast extract 5, NaCl 10, pH 7.0.

[0036] Liquid shake flask fermentation medium (g / L): citric acid 13.5, glutamic acid 10, NH 4 C16.8, Glycerol 75, K 2 HPO 4 1. MgSO4 ·7H 2 O 0.5, FeCl 3 ·6H 2 O 0.02CaCl 2 0.2, MnSO 4 ·H 2 O 0.05, pH 7.2.

[0037] Incline preservation medium (g / L): peptone 10, yeast extract 5, NaCl 10, agar 20, pH 7.0.

[0038] Strain Isolation:

[0039] Weigh 10g of vegetable garden soil sample collected near Xuanwu District, Nanjing City, Jiangsu Province, put it into a triangular flask filled with 90ml of sterile water and glass beads, shake at room temperature at 150 rpm for 30 minutes, and place in a boiling water bath for 3-5 minutes , and...

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Abstract

The invention discloses bacteria YXY-C1 for preparing gamma-polyglutamic acid by solid fermentation and products thereof, and belongs to the technical field of fermentation of microorganisms. In the invention, a bacterial strain YXY-C1 is used for preparing the gamma-polyglutamic acid efficiently by separation; and by using cow dung and monosodium glutamate meal as main raw materials and bran, corn meal, bean meal, citric acid, MgSO4.7H2O and MnSO4.H2O as auxiliary materials, the bacterial strain YXY-C1 can prepare the gamma-polyglutamic acid by solid fermentation, the yield is 39 grams per kilogram of culture medium, and the molecular weight is 300kD. The bacterial strain YXY-C1 can prepare the gamma-polyglutamic acid by fermenting solid wastes and low-price agricultural and sideline products, and has the advantages of low raw material cost, simple method, high yield, convenient product separation and purification and potential application prospect.

Description

1. Technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a bacterium YXY-C1 for producing gamma-polyglutamic acid by solid fermentation and a product thereof. 2. Background technology [0002] γ-polyglutamic acid is a naturally negatively charged and polymerized polymer formed by microorganisms using D- and / or L-type glutamic acid monomers through the amide bond between the α-amino group and the γ-carboxyl group. The polymer, first discovered by Ivanovics and collaborators in the capsule of Bacillus anthracis, has received increasing attention in recent decades. γ-polyglutamic acid has high water solubility and water absorption and moisturizing properties, and is biodegradable, edible, and non-toxic to humans and the environment. Therefore, γ-polyglutamic acid and its derivatives are widely used in food, chemical, and material , medicine and other fields have great development value and broad application ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P13/00C08G69/46C09K17/18G01N35/00G01N27/447C12R1/07
Inventor 沈其荣雍晓雨杨兴明尹成红徐阳春冉炜
Owner 江苏谷荣茂生物科技有限公司
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