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Fungaliasp and immobilizing method and application thereof

A technology of Trametes trichotis and strains, applied in chemical instruments and methods, biochemical equipment and methods, and methods based on microorganisms, etc., can solve the problems of difficult recycling, high cost, and pre-synthesis of carriers, and achieve simple immobilization process , strong decolorization ability and broad industrialization prospects

Inactive Publication Date: 2012-07-18
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently used immobilization carriers are mainly inert materials such as agar, calcium alginate, carrageenan, steel mesh, polymer materials, etc. The decolorization effect is good, but there are carrier needs to be synthesized in advance, the cost is high, and recycling is difficult. question

Method used

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  • Fungaliasp and immobilizing method and application thereof
  • Fungaliasp and immobilizing method and application thereof
  • Fungaliasp and immobilizing method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Example 1. Isolation and identification of Trametes

[0020] Expose the PDA plate containing 0.3mg / ml orange G to the air for 30 minutes, and then incubate it in a constant temperature incubator at 28°C. Observe the occurrence of the decolorization circle, and pick out the hyphae from the decolorization circle; the strain is in the PDA The primary hyphae on the plate are white, and the hyphae turn yellow with time. The PCR amplification and sequencing analysis of the internal transcription spacer sequence of the ribosomal gene in the chromosomal DNA of this strain identified the strain as Fungalia sp. (Fungalia sp.), the strain number is DD616, and this strain has been deposited in the Chinese microbial species The General Microbiology Center of the Deposit Management Committee, the deposit number is CGMCC No. 3680.

[0021] (1) DNA extraction: The DD616 strain was activated on fresh PDA medium and transferred to a shaker flask. After shaking for 3 days, the mycelium was co...

Embodiment 2

[0024] Example 2: Immobilization method of Trametes

[0025] Crush corn cobs, rice straws, wheat straws, reed leaves, etc., weigh 0.5 ~ 5.0g into a 250ml Erlenmeyer flask, then add 5 ~ 15ml water and 0.5 ~ 5.0% glucose into the bottle, and then press and kill at 121°C. After 30 minutes of inoculation, insert 3-8 pieces of trichomonas DD616 strain cultured on PDA medium for 5 days, the diameter of the bacterial cake is 0.7cm, 28℃~30℃ shaker culture for 3~7 days, make The fungus grows on the carrier and removes the culture solution, which is the immobilization of Trametes trichocarpa;

[0026] (1) Decolorization efficiency of different natural carriers: using corn cob, rice straw, reed leaves and wheat straw as carriers, orange G dye as the decolorization object, the decolorization efficiency of immobilized Trametes trichomes DD616 strain for two days is 99%. 99%, 90% and 80%.

[0027] (2) The relationship between fixation time and decolorization: using corncob as the carrier and ora...

Embodiment 3

[0029] Example 3 Application of Trametes Trametes immobilized in the decolorization of printing and dyeing and papermaking wastewater

[0030] (1) De-chromatography: Inoculate the immobilized Trametes Trametes DD616 strain into an Erlenmeyer flask at a dry weight of 30g / L, and add reactive blue K-GC, reactive turquoise blue KN-G, and reactive Red Purple X-2R, Disperse Orange S-4SL, Disperse Blue 2BLN, Orange K-GN, Reactive Red K-2BP, Reactive X Yellow K-6G, Crushed Scarlet SR, 28C shaker culture for 2 days, the decolorization rate is as high as 40~99 %( figure 1) , Showing a good broad spectrum.

[0031] (2) Continuous decolorization efficiency: Continuous decolorization of orange G with immobilized Trametes Trametes DD616 strain, discharge the decolorizing solution every 3 days, continue to add orange G with the same concentration and content, and circulate in turn, the orange G concentration is 0.6g / L; 6 batches of continuous decolorization, a total of 18 days, the decolorizat...

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Abstract

The invention belongs to the technical field of microbial engineering and waste water treatment, and in particular relates to a fungaliasp. DD616, an immobilizing method thereof and application thereof to printing and dyeing and decolorizing of papermaking waste water. The collection number of the strain is CGMCC No. 3680; and the strain is immobilized with maize core, wheat straw, rice straw or reed leaf natural carrier; and the strain can be used for papermaking and decolorizing of the printing and dyeing wastewater and the like after being immobilized.

Description

1. Technical Field [0001] The invention belongs to the technical field of microbial engineering and wastewater treatment, and specifically relates to a Trametes trichome strain and its immobilization method and its application in the decolorization of printing and dyeing wastewater and papermaking wastewater. 2. Background technology [0002] Climate warming and environmental pollution are the most important problems facing mankind in the world today. According to reports, there are 100,000 dyes and 700,000 tons of dyes produced in the world each year. China produces 150,000 tons of dyes each year. In the printing, dyeing and papermaking industries, 10% to 15% of the dyes are discharged with water during the use of dyes. China Approximately 15,000-22,500 tons of printing and dyeing and papermaking wastewater are discharged every year. Printing, dyeing and papermaking wastewater has become one of the most important pollution sources in the natural water ecosystem. [0003] Dyeing a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N11/14C02F3/34C12R1/645C02F103/28C02F103/34
Inventor 刘常宏王兆强薛雅蓉胡杨
Owner NANJING UNIV
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