Melanogenesis inhibitor and skin-whitening agent
A technology of inhibitor and whitening agent, applied in skin care preparations, cosmetic preparations, cosmetic preparations, etc., can solve problems such as unknown melanin effect, and achieve the effect of inhibiting brown spots, strong effect and excellent safety
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[0055] [Melanin Production Inhibition Test]
[0056] For the compound of chemical formula (1), the melanin production inhibitory activity was measured by the following test.
[0057] With the compound of chemical formula (1) as sample, arbutin is used as positive control as sample, hydroxytyrosol, hydroquinone, kojic acid, ascorbic acid 2-glucoside are used as sample as comparison sample, respectively Sulfone was formulated at 10 mg / ml.
[0058] The melanin production inhibitory activity was determined by the following method.
[0059] B16 melanoma F10 derived from mice was inoculated to 20% confluence, and [NLE4, D-PHE7]α-MSH (manufactured by SIGMA-ALDRICH), and the compound of chemical formula (1), arbutin, and hydroxytyrosol were added , hydroquinone, kojic acid, and ascorbic acid 2-glucoside, the concentration of the sample in the culture solution was 1.56 μM to 400 μM. After adding each sample and culturing for 96 hours, the culture solution was removed, and the cells ...
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