Kit for determining mitochondria aspartate aminotransferase
A technology of aspartic acid amino group and aspartic acid, which is applied in the field of mitochondrial aspartate aminotransferase assay kits, can solve the problem of time-consuming determination, difficult automatic determination, and the inability of mitochondrial aspartate aminotransferase to be widely developed and other issues to achieve accurate results
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Embodiment 1
[0019] Example 1 Determination of Mitochondrial Aspartate Aminotransferase
[0020] Use the mitochondrial aspartate aminotransferase assay kit of the present invention to measure mitochondrial aspartate aminotransferase. The kit includes reagent 1 and reagent 2, and both reagent 1 and reagent 2 are liquid preparations. When measuring, the reagent 1 and 2 are used in combination.
[0021] Wherein, the solvent of reagent 1 is water, and its solute is as shown in table 1:
[0022] Table 1. Solutes in Reagent 1
[0023] Tris(Tris)
[0024] L-Aspartic Acid
[0025] The solvent of reagent 2 is water, and the solute is as shown in Table 2:
[0026] Table 2. Solutes in Reagent 2
[0027] Tris
[0028] Samples to be tested: 7 samples of human non-hemolyzed serum or heparin anticoagulated plasma. The mitochondrial aspartate aminotransferase of 7 samples was determined by immunological method, and the results are shown in Table 3.
[0029] Table 3. Resul...
Embodiment 2
[0042] Example 2 Mitochondrial Aspartate Aminotransferase Determination
[0043] Use the mitochondrial aspartate aminotransferase assay kit of the present invention to measure mitochondrial aspartate aminotransferase, the kit includes reagent 1 and reagent 2, and both reagent 1 and reagent 2 are liquid preparations, when measuring Reagents 1 and 2 are used in combination.
[0044] Wherein, the solvent of reagent 1 is water, and its solute is as shown in table 6:
[0045] Table 6. Solutes in Reagent 1
[0046] Tris
[0047] PEG6000
[0048] The solvent of reagent 2 is water, and its solute is as shown in Table 7:
[0049] Table 7. Solutes in Reagent 2
[0050] Tris
[0051] Samples to be tested: 7 human non-hemolyzed serum or heparin anticoagulated plasma samples in Example 1. The method for measuring mitochondrial aspartate aminotransferase activity is the same as the two-step method in Example 1.
[0052] The results after measurement are shown...
Embodiment 3
[0056] Example 3 Determination of Mitochondrial Aspartate Aminotransferase
[0057] The measurement of mitochondrial aspartate aminotransferase uses the mitochondrial aspartate aminotransferase assay kit of the present invention, which kit includes reagent 1 and reagent 2, and reagent 1 and reagent 2 are liquid preparations, when measuring Reagents 1 and 2 are used in combination.
[0058] Wherein, the solvent of reagent 1 is water, and its solute is as shown in table 9:
[0059] Table 9. Solutes in Reagent 1
[0060] Tris
[0061] The solvent of reagent 2 is water, and the solute is as shown in Table 10:
[0062] Table 10. Solutes in Reagent 2
[0063] Tris
[0064] Samples to be tested: 7 human non-hemolyzed serum or heparin anticoagulated plasma samples in Example 1.
[0065] The method for measuring the activity of mitochondrial aspartate aminotransferase is the same as the two-step method in Example 1.
[0066] The results after measurement are sh...
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Abstract
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