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Method for extracting gelatin peptides

An extraction method, the technology of donkey-hide gelatin peptides, applied in the extraction of donkey-hide gelatin peptides, and the field of donkey-hide gelatin peptides obtained by enzymatically hydrolyzing donkey-hide gelatin with biological compound enzymes, can solve the problems of destroying protein components, human body existence, hidden safety hazards, etc., to ensure whiteness and production cycle Effect of short, small molecular weight

Inactive Publication Date: 2012-12-12
湖北远成赛创科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the methods for extracting donkey-hide gelatin peptides mainly include lye extraction and biological enzymatic hydrolysis. The former uses a large amount of alkali treatment in the extraction process, which not only pollutes the environment but also poses a safety hazard to the human body, and also destroys the protein components.
Chinese patent application for the preparation of donkey-hide gelatin low peptide, the publication number CN 101294186A uses pepsin and trypsin for hydrolysis, then ultrafiltration with ultrafiltration column, followed by separation and purification with macroporous adsorption resin, reduced pressure, low temperature concentration, Dried to obtain finished products, the method has cumbersome operation steps and is not suitable for industrial production, and the concentration operation is easy to destroy the product phase, and membrane separation technology is not used, which affects the quality of the product

Method used

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  • Method for extracting gelatin peptides
  • Method for extracting gelatin peptides
  • Method for extracting gelatin peptides

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0020] A kind of extraction method of donkey-hide gelatin peptide, its step is as follows:

[0021] 1. Weigh 1kg of authentic donkey-hide gelatin block (protein 60-80wt%), then add 7kg of distilled water, stir evenly, heat up to 100°C and cook for 8 hours;

[0022] 2. Cool to 55°C, adjust the pH to 7-8 with 0.1M food-grade sodium hydroxide solution, then add 25g alkaline protease and 10g trypsin, stir at 55°C for 10h, during the enzymatic hydrolysis Use the above-mentioned 0.1M sodium hydroxide solution to keep the pH value of the reaction system stable at 7-8; then add 10g of flavor protease, stir at 55°C for 8 hours, and use the above-mentioned 0.1M sodium hydroxide solution to maintain the pH of the reaction system during the enzymolysis process The value is 7-8; then the temperature is raised to 95°C and kept for 15 minutes to inactivate the enzyme;

[0023] 3. Filtrate the enzymatic solution to obtain a supernatant; add 25g of powdered activated carbon to the supernatant...

Embodiment 2

[0028] A kind of extraction method of donkey-hide gelatin peptide, its step is as follows:

[0029] 1. Weigh 2kg of authentic donkey-hide gelatin pieces (protein 60-80wt%), then add 14kg of distilled water, stir evenly, heat up to 100°C and cook for 6 hours;

[0030] 2. Cool to 50°C, adjust the pH to 7-8 with 0.1M food-grade sodium hydroxide solution, then add 20g alkaline protease and 10g trypsin, stir at 50°C for 8h, the enzymolysis process Use the above-mentioned 0.1M sodium hydroxide solution to keep the pH value of the reaction system stable at 7-8; then add 30g of flavor protease, stir at 50°C for 7 hours, and use the above-mentioned 0.1M sodium hydroxide solution to keep the reaction during the enzymolysis The pH value of the system is 7-8; then the temperature is raised to 100°C and kept for 10 minutes to inactivate the enzyme;

[0031] 3. Filtrate the enzymatic solution to obtain a supernatant; add 45g of powdered activated carbon to the supernatant, stir and adsorb ...

Embodiment 3

[0036] A kind of extraction method of donkey-hide gelatin peptide, its step is as follows:

[0037] 1. Weigh 10kg of authentic donkey-hide gelatin (protein 60-80wt%), then add 80kg of distilled water, stir evenly, heat up to 100°C and cook for 7 hours;

[0038] 2. Cool to 55°C, adjust the pH to 7-8 with 0.1M food-grade sodium hydroxide solution, then add 200g alkaline protease and 100g trypsin, stir at 55°C for 7h, the enzymolysis process Use the above-mentioned 0.1M sodium hydroxide solution to keep the pH value of the reaction system stable at 7-8; then add 200g of flavor protease, stir at 55°C for 5 hours, and use the above-mentioned 0.1M sodium hydroxide solution during the enzymolysis process to maintain The pH value of the reaction system is 7-8; then the temperature is raised to 90°C and kept for 20 minutes to inactivate the enzyme;

[0039] 3. Filtrate the enzymatic solution to obtain a supernatant; add 250g of powdered activated carbon to the supernatant, stir and ad...

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Abstract

The invention discloses a method for extracting gelatin peptides. In the method, gelatin is taken as a main raw material; and the method comprises the following steps of: performing heating and boiling with water, enzymolysis with protamex, external cutting with flavourzyme, enzyme inactivation, decoloring and membrane separation, and finally drying obtained concentrated solution to obtain the gelatin peptides. The method provided by the invention ensures a short production cycle, low cost, no toxic and harmful substances and high safety of the obtained product, and can be widely applied in the fields of health care products, medicaments and the like.

Description

technical field [0001] The method of the invention relates to the technical field of deep processing of donkey-hide gelatin, in particular to a method for extracting donkey-hide gelatin peptides, which is a method of using donkey-hide gelatin as a raw material to enzymatically hydrolyze donkey-hide gelatin to obtain donkey-hide gelatin peptides. Background technique [0002] Donkey-hide gelatin is a solid gelatin obtained by decocting and concentrating the dry or fresh hide of equine animal donkey. Sweet and flat in nature and flavor, it belongs to the Lung, Liver and Kidney Meridians, has the functions of nourishing blood, stopping bleeding, nourishing yin and moistening dryness, and is mainly used for blood deficiency syndrome, and can also be used for adjuvant treatment of tumors. Donkey-hide gelatin mainly contains collagen, but the digestion and absorption of collagen requires protease to participate in the degradation, and the indications are common for people with wea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02
Inventor 叶传发张小东胡承四叶思
Owner 湖北远成赛创科技有限公司