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Method for quickly detecting content of glucose in starch fermentation liquor

A technology of starch fermentation and detection method, which is applied in the direction of material analysis by chemical reaction of materials and material analysis by observing the influence on chemical indicators, which can solve the problems of interference detection results, large system, slow detection speed, etc.

Inactive Publication Date: 2013-04-03
HENAN UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Fehling's reagent method has been used for a long time, the measurement results are only approximate, and the operation process is extremely complicated
Like the potassium ferricyanide titration method, both methods require the solution to be titrated in a slightly boiling state. The titration operation is carried out on an electric furnace, and the reaction temperature is not easy to control. The operator's judgment of the titration end point is empirical, and there are many interference factors. , the human error is large, the evaluation of glucose is not accurate enough, and the subjective influence factors are relatively large. Furthermore, when Fehling's solution is used to titrate glucose, other monosaccharides with reducing ability will interfere with the detection results, resulting in large system errors.
The above method is only suitable for laboratory operation, the detection speed is slow, and it is difficult to meet the needs of actual production operations

Method used

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  • Method for quickly detecting content of glucose in starch fermentation liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: glucose standard sample Abs 380 Value - Concentration Standard Curve

[0016] 1. Take a quartz cuvette;

[0017] 2. Use a pipette to accurately pipette 100 μL of standard glucose sample into the cuvette;

[0018] 3. Use a pipette to accurately pipette 2400 μL of self-made glucose optical detection reagent into the cuvette, mix well, and react at room temperature for 1.5 minutes;

[0019] 4. Measure the absorbance value (Abs value) at 380nm under the ultraviolet spectrophotometer;

[0020] 5. Draw glucose standard sample Abs 380 Value - concentration standard curve.

Embodiment 2

[0021] Embodiment 2: the rapid detection method of starch fermentation liquid sample

[0022] 1. Accurately measure 1mL starch fermentation liquid sample and centrifuge;

[0023] 2. Use a pipette to accurately pipette 100 μL of the supernatant into the cuvette;

[0024] 3. Use a pipette to accurately pipette 2400 μL of self-made glucose optical detection reagent into the cuvette, mix well, and react at room temperature for 1.5 minutes;

[0025] 4. Measure the absorbance value (Abs value) at 380nm under the ultraviolet spectrophotometer;

[0026] 5. Compare with the standard curve to determine the content of glucose in the starch fermentation broth sample.

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Abstract

The invention provides a method for quickly detecting the content of glucose in starch fermentation liquor, which is characterized by comprising the following steps of: pretreating a sample, mixing 100mu L of detection sample and 2,400mu L of self-made glucose optical detection reagent, and performing color development reaction on the glucose in the sample and the detection reagent, wherein a reaction product has a maximum absorption peak at 380nm; and making a standard curve by comparing the abs380 (absorbance) values of glucose standard samples with different concentrations, and obtaining the content of the glucose in the substance to be detected according to the abs380 value of the sample to be detected. The detection method has the advantages of high detection accuracy, convenience ofdetection, wide application range and the like, and can be used for quickly detecting the content of the glucose in the starch fermentation liquor.

Description

technical field [0001] The invention relates to a detection technology for rapidly measuring glucose content in starch fermentation liquid, belonging to the field of grain processing detection. Background technique [0002] Glucose is the most common and familiar monosaccharide, also known as dextrose, which belongs to aldols. Glucose is an indispensable nutrient for metabolism in organisms and an important source of energy for human life activities. Glucose can be used directly in the food and pharmaceutical industries. It is used as a reducing agent in the printing and dyeing leather industry. It is often used as a reducing agent in the mirror industry and the silver plating process of thermos bottles. In the industry, a large amount of glucose is used as a raw material to synthesize vitamin C. . In microbial fermentation production, starch is often used as the basic raw material, which is hydrolyzed to produce reducing sugar or glucose for fermentation. Therefore, the d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 何保山李小艳谢玲玲谢岩黎金华丽王金水韩小贤
Owner HENAN UNIVERSITY OF TECHNOLOGY