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Abscisic acid-embedded chitosan nanoparticles and preparation method thereof

A technology of chitosan nano and abscisic acid, which is applied in the field of embedding abscisic acid particles and embedding technology, to prolong the action time, make the production method simple and easy, and improve the effect of disease resistance

Inactive Publication Date: 2013-09-04
GUIZHOU TOBACCO SCI INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no patent application for encapsulating abscisic acid-related or similar microencapsulation technology

Method used

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  • Abscisic acid-embedded chitosan nanoparticles and preparation method thereof
  • Abscisic acid-embedded chitosan nanoparticles and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Weigh 2g of chitosan with a molecular weight of 100kD, add it to 1000mL of acetic acid solution with a mass fraction of 1%, and stir on a magnetic stirrer until fully dissolved; add 10M NaOH dropwise until the pH is 5.5, and the final concentration is 2mg / mL; set aside;

[0022] Dissolve abscisic acid in water to make a concentration of 2g / L abscisic acid aqueous solution for subsequent use;

[0023] Weigh 400mg of sodium tripolyphosphate, add it to 200mL deionized water, stir on a magnetic stirrer until fully dissolved, the pH is about 8.3, and set aside;

[0024] Take 4mL chitosan dilute acetic acid solution with a pH of 5.5, add 0.025mL abscisic acid, mix thoroughly and add 1mL sodium tripolyphosphate solution drop by drop, after fully mixing, embedded nanoparticles can be obtained, and the encapsulation efficiency can be measured was 66%.

Embodiment 2

[0026] Weigh 2g of chitosan with a molecular weight of 50kD, add it to 1% acetic acid solution, stir on a magnetic stirrer until fully dissolved, add 10M NaOH dropwise until the pH is 5.5, adjust the final concentration to 4mg / mL, and set aside;

[0027] Weigh 400 mg of sodium tripolyphosphate, add it to 200 mL of deionized water, stir on a magnetic stirrer until fully dissolved, the pH is about 8.3, and set aside.

[0028] Take 4 mL of dilute acetic acid solution of chitosan with a pH value of 5.5, add 0.025 mL of abscisic acid with a concentration of 2 g / L, mix thoroughly and add 1 mL of sodium tripolyphosphate solution drop by drop, and get embedded nanoparticles after mixing well , The measured encapsulation efficiency was 76%.

[0029] Using the research method of dynamic light scattering, the size dispersion of nanoparticles was measured by PCS particle size analyzer. The test results showed that the chitosan nanoparticles loaded with abscisic acid were monodistributed, ...

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Abstract

The invention discloses abscisic acid-embedded chitosan nanoparticles and a preparation method thereof. The abscisic acid-embedded chitosan nanoparticles are nanoparticles formed by wrapping aqueous solution of abscisic acid by an embedding material which is formed by dilute solution of acetic acid of chitosan and a crosslinking agent. The preparation method comprises the following steps of: adding the chitosan into the dilute solution of acetic acid to be dissolved, and then adding solution of sodium hydroxide to adjust the pH value to obtain dilute solution of acetic acid of the chitosan; preparing abscisic acid into aqueous solution; preparing the crosslinking agent into aqueous solution; adding the solution of dilute acetic acid of the chitosan into the aqueous solution of abscisic acid, and then adding the aqueous solution of crosslinking agent drop by drop; and fully mixing uniformly to obtain the abscisic acid-embedded chitosan nanoparticles. The method is simple and easy, and has high embedding rate; the obtained embedding nanoparticles have no adhesion, good glomeration and round surface; after the embedding, the abscisic acid can be protected from external interference, and the stability of the abscisic acid can be improved; the abscisic acid is steadily and slowly released, which prolongs action time and strengthens induced resistance effect; and the chitosan serving as a wall material has disease-resistant and induced resistance effects.

Description

technical field [0001] The present invention relates to oxygen-containing organic matter, furthermore, relates to abscisic acid, and also relates to embedding technology, especially to particles embedding abscisic acid. Background technique [0002] As we all know, abscisic acid (ABA), also known as S-inducer, is a plant natural growth regulator substance with a sesquiterpene carboxylic acid structure, and is an "anti-stress immune factor" of plants. A large number of researches at home and abroad have proved that S-antigen can activate the stress resistance gene of the plant itself, induce and activate the stress resistance immune system in the plant body, and improve the plant's own resistance to drought, cold, pests, and salinity. The "anti-stress immune factor". As a new type of biological pesticide, abscisic acid can reduce the application of chemical pesticides, improve the yield and quality of agricultural products, protect ecological resources and maintain human hea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N37/42A01N25/28A01P21/00
Inventor 陈兴江商胜华邱雪柏蔡刘体耿召良
Owner GUIZHOU TOBACCO SCI INST
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