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Method for detecting toxicity of characteristic toxic and harmful pollutants of aquatic products

A technology for toxicity detection and pollutants, which is applied in the direction of water testing, food testing, material inspection, etc., can solve the problems of not many screening methods and insufficient basis

Inactive Publication Date: 2013-07-03
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there are not many screening methods for characteristic toxic and harmful pollutants in aquatic products. The traditional methods are mainly historical data retrieval and acute toxicity tests. The basis is not very sufficient and there are many defects.

Method used

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  • Method for detecting toxicity of characteristic toxic and harmful pollutants of aquatic products
  • Method for detecting toxicity of characteristic toxic and harmful pollutants of aquatic products
  • Method for detecting toxicity of characteristic toxic and harmful pollutants of aquatic products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Test the relative toxicity coefficient of different polluted environments

[0021] The specific method is as follows:

[0022] 1. Configure toxic substance solutions with different concentrations to obtain different polluted environments. The relative luminous intensity of Vibrio rascheidi in various polluted environments was determined by using the method of determining the half-lethal concentration of pollutants in a luminescent bacteria toxicity detector. The relative luminous intensity of the bacteria reflects the toxicity of each toxic substance in the polluted environment.

[0023] Taking nine pollutants of Hg, Cr, As, Pb, Cu, Cd, DDT, petroleum, and methyl parathion pesticide (MP) as examples, (there is only one toxic substance in a polluted environment, and each pollutant is Determination) set Cu concentration as 0, 0.1, 0.2, 0.3, 0.5, 1 mg / L; Pb concentration as 0, 1, 2, 3, 4, 5 mg / L; Cd concentration as 0, 0.1, 0.5, 0.7, 1mg / L; Hg concentrations are 0, 0.1,...

Embodiment 2

[0031] Test the pollution of algae products by various toxic substances

[0032] See Table 2 below for the enrichment coefficient BCF of kelp, P.

[0033] Table 2 The enrichment factor BCF of various pollutants in algae

[0034]

[0035] 1, in conjunction with the relative toxicity coefficient among the embodiment 1, judge the pollution situation of each toxic substance to sea-tangle:

[0036] The ratio of relative toxicity coefficient of Cd to BCF: 0.720 / 790=9.11×10 -4

[0037] The ratio of relative toxicity coefficient of Cu to BCF: 0.541 / 360=1.50×10 -3

[0038] The ratio of relative toxicity coefficient of Pb to BCF: 2.813 / 140=2.01×10 -2

[0039] The ratio is: CdCu>Pb

[0040] 2, in conjunction with the relative toxicity coefficient of each material among the embodiment 1, judge the pollution situation of each toxic material to subcardiac flat algae:

[0041] The ratio of relative toxicity coefficient of Cd to BCF: 0.720 / 17642.61=4.08×10 -5

[0042] The ratio o...

Embodiment 3

[0056] Test the contamination of shellfish products by various toxic substances

[0057] The enrichment factor BCF of each shellfish product is shown in Table 3 below.

[0058] Table 3 The enrichment factor BCF of various pollutants in shellfish

[0059]

[0060] The order of toxicity of each toxic substance to razor clams:

[0061] The ratio of relative toxicity coefficient of As to BCF: 4.966 / 371=0.0134

[0062] The ratio of relative toxicity coefficient of Hg to BCF: 0.358 / 429630=8.333×10 -7

[0063] The ratio of relative toxicity coefficient of Cd to BCF: 0.720 / 3679=0.0002

[0064] The ratio of relative toxicity coefficient of Pb to BCF: 2.813 / 3=0.9377

[0065] Relative toxicity coefficient of Cu to BCF ratio: 0.541 / 170=0.0032

[0066] The ratio of relative toxicity coefficient of Cr to BCF: 1.043 / 4=0.2608

[0067] The ratio is: HgCd>Cu>As>Cr>Pb

[0068] The order of toxicity of various toxic substances to cockles:

[0069] The ratio of relative toxicity coeffi...

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Abstract

The invention relates to a method for detecting the toxicity of characteristic toxic and harmful pollutants of aquatic products. The method is characterized by comprising the following steps of: (1) measuring the relative luminous intensity (RL) of luminous bacteria in a polluted environment of toxic substances with different concentrations by adopting a half lethal concentration method in a toxicity detection analyzer; (2) solving a linear regression equation, i.e. Y=aX+6, through regression analysis by taking the concentrations of various toxic substances in the polluted environment as a horizontal coordinate and the RL as a vertical coordinate, solving the diluted concentration, i.e. an EC50 value, of a corresponding pollutant when the RL is 50%, and defining the EC50 value as a relative toxicity factor (RT); (3) testing the BCF (Biological Concentration Factor) of the various toxic substances in the polluted environment; and (4) carrying out division on the obtained RT and the BCF, wherein an obtained value is the absolute toxicity magnitude of the various toxic substances in the polluted environment. The detection method provided by the invention can be used for accurately and fast sequencing the toxicity of various pollutants on the aquatic products.

Description

technical field [0001] The invention relates to a toxicity detection method for characteristic toxic and harmful pollutants of aquatic products. Background technique [0002] In recent years, with the rapid development of the coastal economy, the discharge of industrial wastewater, waste and pollutants containing heavy metals to the marine environment has continued to increase. The ecological environment has also caused varying degrees of damage. my country's marine aquaculture industry is developing rapidly and on a large scale, and is increasingly affected by environmental pollution. The aquaculture environment has been seriously polluted, so that pollutants accumulate in aquatic products, and these pollutants enter the human body through the food chain and will affect human health. Screen the characteristic toxic and harmful pollutants of aquatic products, determine the primary and secondary pollution of toxic and harmful pollutants to aquatic products, and the priority...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/18G01N33/02
Inventor 徐永健葛奇伟陆慧贤孙彬戴广谱卢光明
Owner NINGBO UNIV
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