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Two-aqueous-phase method for extracting total saponin from bittersweet herb

A two-phase, total saponin technology, applied in the field of medicine, can solve problems such as complex pretreatment methods, cumbersome operations, and solvent toxicity, and achieve the effects of easy mastery, low interfacial tension, and simple methods

Inactive Publication Date: 2012-05-30
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using n-butanol extraction, the solvent used is highly toxic, and needs to be repeated three times, and the operation is cumbersome; using macroporous adsorption resin, the pretreatment method is complicated, and the extraction rate is low, time-consuming

Method used

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  • Two-aqueous-phase method for extracting total saponin from bittersweet herb
  • Two-aqueous-phase method for extracting total saponin from bittersweet herb
  • Two-aqueous-phase method for extracting total saponin from bittersweet herb

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Solid-liquid extraction

[0047] Take about 1.0 g of the pulverized Baiying medicinal material, weigh it accurately, extract in a 90°C water bath with 70% ethanol under reflux for 1 hour, repeat 3 times and then filter, heat and concentrate the filtrate to obtain an extract, disperse it with water to the original filtrate 1 / 5 of the volume to obtain the Baiying sample solution.

[0048] (2) Aqueous two-phase extraction

[0049] Take the above-mentioned Baiying sample solution, add anhydrous ethanol / ammonium sulfate two-phase system, wherein the mass fraction of absolute ethanol is 25%, the mass fraction of ammonium sulfate is 17%, the pH is 5.8, place at 30°C for 10 minutes, and let stand Phase. Wherein the yield of total saponins in absolute ethanol phase is 76.8%.

[0050] (3) Take out the absolute ethanol phase, place it at 70°C for distillation under reduced pressure, and dry it in vacuum to obtain the total saponins extract of Baiying.

Embodiment 2

[0052] (1) Solid-liquid extraction

[0053] Take about 1.0 g of the pulverized Baiying medicinal material, weigh it accurately, extract in a 90°C water bath with 70% ethanol under reflux for 1 hour, repeat 3 times and then filter, heat and concentrate the filtrate to obtain an extract, disperse it with water to the original filtrate 1 / 5 of the volume to obtain the Baiying sample solution.

[0054] (2) Aqueous two-phase extraction

[0055] Take the above-mentioned Baiying sample solution, add anhydrous ethanol / ammonium sulfate two-phase system, wherein the mass fraction of absolute ethanol is 25%, the mass fraction of ammonium sulfate is 22%, the pH is 5.8, place at 30°C for 10 minutes, and let stand Phase. Wherein the yield of total saponins in absolute ethanol phase is 77.0%.

[0056](3) Take out the absolute ethanol phase, place it at 75°C for vacuum distillation, and vacuum dry to obtain the total saponins extract of Baiying. Example 3:

Embodiment 3

[0057] (1) Solid-liquid extraction

[0058] Take about 1.0 g of the pulverized Baiying medicinal material, weigh it accurately, extract in a 90°C water bath with 70% ethanol under reflux for 1 hour, repeat 3 times and then filter, heat and concentrate the filtrate to obtain an extract, disperse it with water to the original filtrate 1 / 5 of the volume to obtain the Baiying sample solution.

[0059] (2) Aqueous two-phase extraction

[0060] Take the above-mentioned Baiying sample solution, add anhydrous ethanol / ammonium sulfate two-phase system, in which the concentration of absolute ethanol is 16%, the concentration of ammonium sulfate is 20%, the pH is 5.8, stand at 30°C for 10 minutes, and stand still for phase separation. Wherein the yield of total saponins in absolute ethanol phase is 65.4%.

[0061] (3) Take out the absolute ethanol phase, place it at 80° C. for vacuum distillation, and vacuum dry to obtain the total saponins extract of Baiying.

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Abstract

The invention belongs to the technical field of medicines, and relates to a two-aqueous-phase method for extracting total saponin from bittersweet herb. The Two-aqueous-phase method for extracting total saponin from bittersweet herb is characterized by comprising the following steps of: pulverizing bittersweet herb, adding to a solvent, carrying out solid-liquid digestion and then filtering for impurity removal to obtain a coarse extract of the bittersweet herb; concentrating the coarse extract of the bittersweet herb to 1 / 5 of the original volume, adding to a two-aqueous-phase system formed from absolute ethyl alcohol / ammonium sulfate, adding water to ensure that the total amount of the system is a certain value, uniformly mixing and then standing to form an upper phase and a lower phase; and taking out the absolute ethyl alcohol phase containing a great amount of total saponin of the bittersweet herb, and then carrying out pressure-reduction concentration on the absolute ethyl alcohol phase obtain a total saponin extractive of the bittersweet herb. The two-aqueous phase method has the beneficial effects that the extraction rate of the total saponin of the bittersweet herb is improved; as the used solvent has low toxicity, the method is beneficial to environmental protection; and simultaneously the method has convenience and economy.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a method for extracting total saponins in bletilla radix with two aqueous phases. Background technique [0002] Solanaceae Solanum lyratum The dried whole plant of Thunb., also known as white hair rattan, is mainly produced in Jiangsu, Zhejiang, and Jiangxi. It is slightly cold in nature and bitter in taste. It was first seen in the records of "The Classic", listed as top grade, and is a traditional Chinese medicine. It has the effects of clearing heat and detoxifying, dispelling wind and reducing phlegm, and dehumidification. It is used to treat diseases such as cancer, herpes, malaria, jaundice, edema, gonorrhea, rheumatoid arthritis, and abnormal leucorrhea. Baiying contains a variety of chemical components, mainly steroidal saponins, steroidal alkaloids, organic acids and other compounds. Among them, the saponins mainly include various steroidal saponins with diosgenin, tyc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/81A61P3/06A61P7/02A61P9/00A61P35/00A61P37/02
Inventor 孙立新贾玉荣
Owner SHENYANG PHARMA UNIVERSITY
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