Pulp capping agent with bioactivity and preparation method thereof

Abstract

The invention discloses a pulp capping agent with bioactivity and a preparation method thereof. The pulp capping agent with bioactivity comprises dentin extraction solution and dentin powder. The pulp capping agent preparation method comprises the following steps: (1) selecting health dental tissue, cleaning by PBS (Phosphate Buffer Solution) to remove periodontal ligament, cementum and dental pulp, and sterilizing; (2) freezing the dental tissue, and grinding the frozen dental tissue to particles; (3) culturing the dental tissue particles in a medium; and (4) filtering the supernatant from the step (3) to obtain the dentin extraction solution, centrifugally cleaning the cultured dentin particles by the PBS, oven-drying, and grinding to obtain the dentin powders. The pulp capping agent can effectively promote the repair of dentin only by blending the dentin extraction solution with the dentin powder, and applying the mixture at a position close to the dental pulp or at a position at which the dental pulp is exposed, thereby improving the success rate of live-marrow conservation, and providing a new treatment means for patients suffering from accident pulp perforation.

Description

technical field [0001] The invention belongs to a novel preparation method of a tooth restorative material with biological activity, and in particular relates to a method for constructing a biological active material after treatment of dental tissues of various animals including humans. technical background [0002] At present, dental caries has become a major disease that endangers the oral cavity and even human health. WHO has listed dental caries as the third disease that endangers human health, and its incidence rate is increasing year by year. The light ones cause cold and hot stimulation discomfort, and the severe ones cause spontaneous pain, which is severe and seriously affects the patient's physical and mental health, mood and work. Further development of the lesion can cause pulp disease, periapical disease, and jaw osteomyelitis, seriously affecting the patient's general health. In addition, if dental caries is allowed to develop, it will eventually lead to the l...

AI Technical Summary

Problems solved by technology

However, clinically, the long-term success rate of pulp surgery is not very high. Studies have shown that the 5-year success rate of Ca(OH)2 direct pulp capping is 45%, while the 10-year success rate is only 20% (M. Goldberg, N. Six, C. Chaussain, et al, Dentin Extracellular Matrix Molecules Implanted into Exposed Pulps Generate Reparative Dentin: a Novel Strategy in Regenerative Dentistry, J Dent Res. 2009 May ; 88(5): 396 –399)

For cases where the pulp capping agent fails, further root canal treatment is required. When the pulp capping agent is Ca(OH)2, Ca(OH)2 may exist Restricted induced mineralization, the patient's pulp cavity and root canal cavity are severely calcified, making it difficult to complete root canal treatment, and clinical treatment is time-consuming and laborious

[0004]Although the Ca(OH)2 pulp capping agent has the above-mentioned deficiencies, there is no substitute for calcium hydroxide that can form a repair dentin or bioactive pulp capping agent

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