Preparation method of low-molecule brown seaweed glucan

A technology of glucan and low molecular weight, applied in the field of preparation of low molecular fucoidan, can solve the problems such as the lack of large-scale production and application of fucoidan

Active Publication Date: 2013-01-16
北京雷力联合海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country is a large brown algae breeding country in the world, with an estimated annual output of more than 80,000 tons. At present, the production scale of alginate in my country is very large, and it has been widely used in food processing. The production of fucoidan started relatively late, but it has developed rapidly and has been applied For health food or medicine, there is no large-scale production and application of algae starch

Method used

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Examples

Experimental program
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Effect test

example 1

[0014] (1) Preparation of algal starch

[0015] Take 100g and crush to 1~5cm 2 Put the kelp into 2000mL solution containing 30g of sodium carbonate, keep at 80℃ and stir for 0.5 hours to disintegrate the tissues and cells of kelp, slowly add hydrochloric acid to neutralize to pH 4, and then extract for 2 hours; centrifuge or filter, Discard the centrifugal residue or filter residue (which can be used to produce seaweed fertilizer), and pass the clear liquid through an ultrafiltration membrane or hollow fiber ultrafiltration column with a molecular weight cut-off of 3000 Daltons, and concentrate to 220mL, add 100mL of absolute ethanol to precipitate the alginic acid ; Then add 250mL of absolute ethanol to 300mL of centrifugal solution to precipitate algin and fucoidan, centrifuge to separate the precipitate, and dehydrate and dry to obtain algin and fucoidan; then continue to add absolute ethanol to 530mL of centrifuged solution, Make the ethanol concentration reach 85%, centrifug...

example 2

[0019] (1) Preparation of algal starch

[0020] Take 200g of wakame crushed to 20 mesh, put it into 5000mL solution with 50g potassium carbonate, stir and extract at 80℃ for 0.5 hours, neutralize with sulfuric acid to pH 2, continue to stir and extract for 4 hours, centrifuge or filter, discard the centrifuge Residue or filter residue (can be used to produce seaweed fertilizer), the clear liquid passes through an ultrafiltration membrane or hollow fiber ultrafiltration column with a molecular weight cut-off of 3000 Daltons, and is concentrated to 600mL, and 250mL of absolute ethanol is added to precipitate the alginic acid; Add 620mL of absolute ethanol to 810mL of centrifugal liquid to precipitate algin and fucoidan, centrifuge the precipitate, and dehydrate and dry to obtain algin and fucoidan; then continue to add absolute ethanol to 1400mL of centrifugal liquid to make the ethanol concentration Reaching 80%, centrifugal separation and precipitation, dehydration and drying to ...

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Abstract

The invention belongs to a preparation method of low-molecule brown seaweed glucan. Alkali carbonate solution is used for breaking up seaweed tissue and cells, acid is used for adjusting a pH value to be proper, alginic acid, brown seaweed polysaccharide and brown seaweed starch are extracted from the seaweed tissue and the cells, and the starch is degraded with hydroxide to turn into the brown seaweed glucan with the average molecular weight being 800-1000 Dalton. As reported, the brown seaweed glucan with the low molecular weight can enhance the immunity of a human body and the stress resistance of plants.

Description

Technical field [0001] A preparation method of seaweed extract. technical background [0002] Seaweed is one of the most abundant resources in the world. In particular, large brown algae such as kelp and wakame have been successfully cultivated and the output has greatly increased. It is estimated that the world's kelp production exceeds 200,000 tons. my country is the world’s largest brown algae farming country, with an estimated annual output of more than 80,000 tons. At present, the production scale of algin in my country is very large and has been widely used in food processing. The production of fucoidan started late, but it has developed rapidly and has been applied For health food or medicine, algal starch has no large-scale production and application. According to reports, both kelp and wakame contain brown algae starch, the content is about 1%, and is composed of β-1,3 structure glucose, but also contains a small amount of β-1,6 structure and a small amount of mannose. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02A01P21/00
Inventor 汤洁
Owner 北京雷力联合海洋生物科技有限公司
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