Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nanoparticle gene delivery system of cationized lycium barbarum polysaccharides and preparation method thereof

A gene delivery system, the technology of Lycium barbarum polysaccharide, which is applied in nanotechnology, nanomedicine, nanotechnology and other directions, can solve the problems of limited loading capacity, limitation and high cost, and achieve good proliferation, good encapsulation and release effect, and good gene transfer. expressive effect

Active Publication Date: 2011-08-17
JIANGSU UNIV
View PDF2 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the former has higher transfection efficiency, its induction of host immune response, potential tumorig

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nanoparticle gene delivery system of cationized lycium barbarum polysaccharides and preparation method thereof
  • Nanoparticle gene delivery system of cationized lycium barbarum polysaccharides and preparation method thereof
  • Nanoparticle gene delivery system of cationized lycium barbarum polysaccharides and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0043] Preparation of refined Lycium barbarum polysaccharide

[0044]Extraction and purification of polysaccharides: take medlar, crush, water extraction and alcohol precipitation (according to the following process hot water extraction: material-liquid ratio: 1:5~20, extraction temperature: 50~80°C, extraction time 0.5~4h / time , Extraction times: 2 times. After combining the two extracts, concentrate by rotary evaporation to 1 / 5-1 / 10 of the volume of the original extract, and then add 95% ethanol to the concentrated solution until the final concentration of ethanol is 65% -85%), freeze-dried to obtain crude polysaccharides from Lycium barbarum, removed protein by trichloroacetic acid method, and dialyzed (cut-off molecular weight>3500Da); successively used DEAE-52 cellulose resin (eluent: double distilled water and 0.05~0.5mol / LNaCl) and SephadexG-100 dextran gel resin (eluent: 0.1mol / LNaCl) were separated and purified, and the molecular weight distribution of the isolated...

Embodiment 1

[0045] Embodiment one, Preparation of oxidized Lycium barbarum polysaccharide

[0046] Take 0.2g refined Lycium barbarum polysaccharide, dissolve it in 30ml double distilled water, add 0.3g KIO 4 , quickly placed in a dark room, magnetically stirred, and reacted at room temperature for 72 hours; the reaction solution was terminated by adding 15ml of ethylene glycol, and the reaction was continued for 30 minutes according to the above conditions; Lycium barbarum polysaccharide 0.15g.

Embodiment 2

[0047] Embodiment two , Preparation of gene delivery system of ethylenediamine-modified Lycium barbarum polysaccharide-DNA plasmid nanocomposite

[0048] Take 0.1g oxidized wolfberry polysaccharide and dissolve it in 20ml double distilled water; weigh 0.14ml ethylenediamine and dissolve it in 5ml borate buffer solution (pH=9); slowly drop the borate solution containing ethylenediamine into oxidized wolfberry In the polysaccharide solution, magnetic stirring was carried out at the same time; after the addition, magnetic stirring was carried out, and the reaction was carried out at room temperature for 24 hours; after that, 0.1 g sodium borohydride was added to the reaction solution, and the reaction was continued for 48 hours under the same conditions; then 0.1 g sodium borohydride was added to the reaction solution sodium, and continued to react for 24 hours under the same conditions; the collected reaction solution was dialyzed in double-distilled water for 48 hours, with a ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Number average molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention relates to a nanoparticle gene delivery system of cationized lycium barbarum polysaccharides, in particular to a gene delivery system of lycium barbarum polysaccharide-DNA (deoxyribonucleic acid) plasmids modified by using an aminated compound. The molecular weight distribution of lycium barbarum polysaccharides is 20-40KD, wherein the mass ratio of the cationized lycium barbarum polysaccharides to the DNA plasmids is (1-200):1; the particle size of a cationized lycium barbarum polysaccharide-DNA plasmid compound is 351-414 nanometers; and the aminated compound is spermine, ethylene diamine or polyethylene imine with the number average molecular weight of 600-2,000Da. An electrophoresis experiment and a stem cell transfection experiment indicate that three types of cationized lycium barbarum polysaccharides have good DNA plasmid combining functions and genetic transmission expression functions. Meanwhile, an extensive application basis is laid for the gene delivery system of the cationized lycium barbarum polysaccharides by using an appropriate molecular particle size, biodegradability and immunogenicity resistance.

Description

technical field [0001] The invention relates to a wolfberry polysaccharide and a gene transfer system, in particular to a cationized wolfberry polysaccharide nanoparticle gene transfer system. Background technique [0002] Gene therapy refers to the introduction of exogenous normal genes into target cells to correct or compensate for diseases caused by gene defects and abnormalities, so as to achieve the purpose of treatment. The key to the success of gene transfer technology is to effectively transfer genetic material into cells and make it efficiently participate in the replication and expression of genetic material in targeted cells. [0003] Gene delivery system (gene delivery system, GDS) plays a very important role in gene therapy. Usually GDS is divided into two categories: viral vectors and non-viral vectors. Although the former has a higher transfection efficiency, its induction of host immune response, potential tumorigenicity, limited loading capacity, and high ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/63B82Y5/00
Inventor 徐希明王淼余江南邓纹纹曹霞
Owner JIANGSU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com