Taxus polysaccharide immunologic adjuvant and influenza vaccine containing same
A technology of yew polysaccharides and immune adjuvants, applied in the field of influenza vaccines, can solve the problems of limited vaccine production and supply, difficulties in meeting infectious diseases, and unavoidable side effects, and achieve weakened activity, high safety, and local swelling of injections obvious effect
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[0043] Example 1
[0044] Ethanol Extraction of Taxus chinensis Polysaccharide
[0045] Take 500g of dried Mandia yew branches and leaves, pulverize them into powder, and place them in a container. Use 4 times the volume of 2000 ml ethanol with a concentration of 95% to perform heating and reflux extraction as usual, where each heating temperature is 50°C The reflux time is 60 minutes, and the number of extractions is 2 times. After filtration, the filtrate is decompressed to recover ethanol. After the remaining filtrate is concentrated and dried, 50 grams of ethanol extract is obtained. The paclitaxel content of the extract is 0.2%. The content is more than 60%. Mix the material with 200 mesh silica gel G, load it into a glass chromatography column with an inner diameter of 15 cm and a height of 150 cm. Use methanol and chloroform (the volume ratio of the two is 1:1) as the mobile phase for routine After elution, the yew polysaccharide can be seen brownish-red in the glass column...
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[0046] Example 2
[0047] Water extraction of yew polysaccharide
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[0049] Example 3
[0050] Detection of Mandia yew polysaccharides: Take the Mandia yew polysaccharides obtained in Example 1 and Example 2, respectively, according to the method of "The Pharmacopoeia of the People's Republic of China" (2010 edition), page 1008, China Medical Science and Technology Press, High performance liquid chromatography is used for detection, and its indicators meet the requirements of polysaccharides.
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