Method for freezing and embedding tissue paraffin section

Abstract

The invention discloses a method for freezing and embedding a tissue paraffin section. The method comprises the following steps of: placing a paper embedding frame impregnated with paraffin tissues and molten paraffin into a 4 DEG C refrigerating chamber, standing for 10 minutes, peeling off paraffin blocks from the packet-embedding frame, slicing and dyeing. Compared with the conventional cold water embedding method of the tissue paraffin section, the method disclosed by the invention is simple, fast, environment-friendly and practical; the paper embedding frame can be recycled; cold water is not required, and phenomenon such as the formation of a hollow because water enters the paraffin block is avoided; and the quality of the section is guaranteed. Moreover, the method is wide in application, can be used for tissue sections of animals and plants and pathological sections of human bodies.

Description

【Technical field】 [0001] The invention discloses a refrigerated embedding method for paraffin tissue sections, relates to the technical field of biological and pathological tissue sections, and is a method for embedding tissue paraffin sections with the characteristics of simplicity, quickness, environmental protection and practicality. 【Background technique】 [0002] Tissue paraffin section is the most commonly used sectioning technique for observing tissue morphology and structure, and is widely used in biological science research and clinical pathological diagnosis. The operation process generally includes tissue fixation, dehydration, clearing, paraffin dipping, embedding, slicing, sticking, spreading, dewaxing, rehydrating, staining, dehydrating, clearing, and sealing. The method of embedding is usually to pour melted paraffin into a paper embedding frame, wait for the paraffin to condense on the bottom layer, put the tissue soaked in wax, and place it in a correct posi...

AI Technical Summary

Problems solved by technology

[0003] The disadvantages of this method are: 1. The paper embedding frame is disposable and cannot be reused after soaking in water, which is not environmentally friendly.

2. Cold water needs to be prepared before embedding, which is time-consuming and laborious

3. The wax blocks are easy to enter water, forming cavities, resulting in waste products, seriously affecting the quality of slices and even the success or failure of experiments.