Primer composition, kit and method for detecting mutation of exon 19 of human EGFR gene

Abstract

The invention belongs to the fields of biotechnology and medical science, and particularly relates to a primer composition, a kit and a method for detecting mutation of an exon 19 of a human epidermal growth factor receptor (EGFR) gene. The primer composition comprises a primer for detecting the mutation of the exon 19 of the human EGFR gene and an upstream primer SEQ ID NO:2 inside the exon 19 of the human EGFR gene, wherein a nucleotide sequence of an upstream primer for detecting the exon 19 of the human EGFR gene is SEQ ID NO:1, a nucleotide sequence of a downstream primer is SEQ ID NO:3. In the primer composition, the mutation of the exon 19 of the human EGFR gene is used as a detection object, and the detection of microfluidic chips is performed on polymerase chain reaction (PCR) products by utilizing a heminested PCR reaction of a special primer group so as to diagnose the mutation of the human EGFR gene quickly, simply, accurately and sensitively.

Description

technical field [0001] The invention belongs to the fields of biotechnology and medicine, in particular, the invention relates to a primer composition, a kit and a method for detecting mutations in exon 19 of human EGFR gene. Background technique [0002] Lung cancer is a relatively common primary malignant tumor of the lung, with high morbidity and mortality. Male lung cancer accounts for the first cause of various cancer deaths, and female is second only to breast cancer. According to the traditional histopathological classification, lung cancer can be divided into small cell lung cancer and non-small cell lung cancer. Non-small-cell lung cancer (NSCLC) is "non-small cell carcinoma", including squamous cell carcinoma, adenocarcinoma, and large cell carcinoma. Compared with small cell carcinoma, its cancer cells grow and divide slower, and their diffusion and metastasis are relatively slow Later, non-small cell lung cancer accounts for about 80-85% of the total lung cancer...

AI Technical Summary

Problems solved by technology

Compared with DNA sequencing, although other molecular biology methods have improved sensitivity and / or specificity, most methods still have higher requirements for tissue sampling, requiring more tumor tissues and higher tumor cell content

In addition, some methods, such as fluorescent real-time polymerase chain reaction, high performance liquid chromatography and capillary electrophoresis, require special equipment, so they still cannot be widely promoted in clinical practice.

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