Standard molecule simultaneously suitable for specificity detection on seven transgene rape strains

A specific, transgenic technology applied in the biological field

Active Publication Date: 2011-10-12
SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there has been no report on constructing standard molecul...

Method used

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  • Standard molecule simultaneously suitable for specificity detection on seven transgene rape strains
  • Standard molecule simultaneously suitable for specificity detection on seven transgene rape strains
  • Standard molecule simultaneously suitable for specificity detection on seven transgene rape strains

Examples

Experimental program
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Effect test

Embodiment 1

[0150] Embodiment 1, standard molecular construction

[0151] In order to construct general standard molecules suitable for the specific detection of transgenic rapeseed RT73, MS1×RF1, MS1×RF2, MS8×RF3, T45, Oxy235 and Topas19 / 2 lines, MS1-5, RT73-3, RF3-3, RF3 -5, MS8-3, MS8-5, Oxy235-5, Oxy235-3, RF1-5, T45-5, Topas19 / 2-3, RF2-5, HMG, PEP and other 14 specific sequence fragments were gradually cloned into In the pEASY-T3 vector, pCanola9 ( figure 1 ), the size is about 7921bp. The results of three times of plasmid whole gene sequencing were consistent with the target sequence.

[0152] MS1-5 (SEQ ID NO: 1, 299bp):

[0153] GGCCTGTGGTCTCAAAGATGGATCATTAATTTCCACCTTCACCTACGATGGGGGGCATCGCACCGGTGAGTAATATTGTACGGCTAAGAGCGAATTTGGCC TGTAGACCTCAATTGCGAGCTTTCTAATTCAAACTATTCGGGCCTAACTTTTGGTGTGATGATGCTGAAGAACCTATCCATGAAACTCACAAAAACATC

[0154] RT73-3 (SEQ ID NO: 2, 316bp):

[0155] CGACGGATCGTAATTTGTCGTTTTATCAAAATGTACTTTCATTTTATAATAACGCTGCGGACATCTACATTTTTGAATTGAAAAAGAATTGGTAATTACTCTT...

Embodiment 2

[0182] Embodiment 2, standard molecule is used for the applicability identification of ordinary PCR detection

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Abstract

The invention relates to standard molecule simultaneously suitable for specificity detection on seven transgene rape strains. A universal type standard molecule simultaneously suitable for specificity detection on a rape an RT73 strain, an MS1*RF1 strain, an MS1*RF2 strain, an MS8*RF3 strain, a T45 strain, an Oxy235 strain and/or a Topas19/2 strain is constructed to carry out verification on the applicability of the standard molecule for qualitative and quantitative detection. The standard molecule is used for highly detecting the specificity of the transgene rape strains.

Description

technical field [0001] The invention belongs to the field of biotechnology; more specifically, the invention relates to a standard molecule suitable for the specific detection of seven transgenic rapeseed lines at the same time. Background technique [0002] In recent years, genetically modified organism technology has been widely used in agricultural production. Countries around the world are constantly improving the research on detection methods of genetically modified organisms and strengthening the safety supervision of genetically modified raw materials and their products. Among them, nucleic acid detection technology has become the most important and commonly used method for the detection of genetically modified organisms. Under the existing technical conditions, the exogenous gene is inserted into the crop genome in a random manner, so the boundary sequence between the exogenous insertion fragment and the DNA binding site of the crop genome in each transgenic crop var...

Claims

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Application Information

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IPC IPC(8): C12N15/63C12Q1/68
Inventor 李想潘良文吕蓉高琴刘月明张舒亚
Owner SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
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