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Markers of induced pluripotent stem cells

A technology of pluripotent stem cells and cells, applied in the application field of labeling, can solve the problem of no observed tumor formation, etc.

Inactive Publication Date: 2011-10-12
AGENCY FOR SCI TECH & RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Importantly, no tumor formation was observed when hESCs were treated with mAb 84 prior to transplantation into SCID mice

Method used

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  • Markers of induced pluripotent stem cells
  • Markers of induced pluripotent stem cells
  • Markers of induced pluripotent stem cells

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0305] Materials and Methods

[0306] cell culture

[0307] The human embryonic stem cell line HES-3 was obtained from ES Cell International. at 37°C / 5% CO 2 Cells were cultured on artificial basement membrane-coated organ culture dishes supplemented with conditioned medium from a mouse feeder, ΔE-MEF (feeder-free culture). The medium used for culturing hESCs is KNOCKOUT (KO) medium. as previously stated 4 , hESCs were passaged after enzyme treatment for feeder-free cultures.

[0308] Human induced pluripotent stem cell lines, ES4SKIN and ESIMR90, were obtained from the University of Wisconsin and as previously described 4 To cultivate. The human foreskin fibroblast cell line, BJ1 (CRL-2522), and the lung fibroblast cell line, IMR90 (CCL-186), were purchased from American Type Culture Collection and cultured according to the suppliers' instructions.

[0309] Flow Cytometry Analysis

[0310] Using trypsin, cells were harvested as a single-cell suspension at 2 x 10 5 ce...

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Abstract

The disclosure relates to the expression of podocalyxin-like protein (PODXL) on the surface of induced pluripotent stem cells and particularly, although not exclusively, to the use of PODXL as a marker of induced pluripotent stem cells.

Description

technical field [0001] The present invention relates to the expression of podocalyxin-like protein (PODXL) on the surface of induced pluripotent stem cells, and particularly, though not exclusively, to the use of PODXL as a marker for induced pluripotent stem cells. Background technique [0002] Embryonic stem cells and other pluripotent stem cells have great potential in therapy. The above-mentioned cells can be used in regenerative medicine to repair tissues damaged by disease or injury. However, the use of embryonic stem cells in medicine is limited due to important ethical issues associated with the use of human embryos. Recently, Yamanaka Lab 2 and Thomson Lab 3 showed that by transient overexpression of a few genes, human fibroblasts can be reprogrammed into induced pluripotent stem cells (IPSCs), which are functionally and phenotypically similar to hESCs. Therefore, pluripotent stem cells can be obtained without destroying embryos. [0003] These IPSCs are functi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00A61K35/12G01N33/53
CPCA61K35/12C07K14/705C12N2501/50C12N2506/45A61P17/00A61P19/00A61P25/14A61P25/16A61P25/28A61P35/00A61P9/04A61P3/10
Inventor 徐文华胡家荣
Owner AGENCY FOR SCI TECH & RES