Expression vector, and its construction and application

A technology of expressing vectors and linearized vectors, which is applied in the field of molecular genetics, can solve problems such as inconvenience, and achieve the effect of increasing safety

Inactive Publication Date: 2011-10-26
XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] It can be seen that the above-mentioned existing expression vectors obviously still have inconvenience and defects in structure and use, and need to be further improved urgently.

Method used

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  • Expression vector, and its construction and application
  • Expression vector, and its construction and application
  • Expression vector, and its construction and application

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Embodiment Construction

[0056] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments, but not as a limitation of the present invention.

[0057] figure 1 It is a structural diagram of the expression vector of the present invention, in which the names and positions of each element are marked; figure 2 It is the structural diagram after the expression vector of the present invention is connected with the target gene and linearized. Such as figure 1 with figure 2 As shown, an expression vector, the Loxp sequence is added at both ends of the marker gene of the linearized vector and the non-target gene of the resistance gene, and an inducible expression promoter and TK gene components are introduced at the same time. The expression vector of the present invention specifically includes the following parts:

[0058] Ptight (bp 33-348) selective promoter, jointly initiated by Tet-On Advanced protein subunit and tetracyclin...

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Abstract

The invention discloses an expression vector, and its construction and application. The construction of the expression vector comprises adding LoxP sequences at two ends of an untargeted gene of a linear vector and introducing inducible expression Cre enzyme and TK suicide gene components simultaneously. The expression vector improves securities of transgenic animals.

Description

technical field [0001] The invention relates to the technical field of molecular genetics, in particular to an expression vector and its construction and application. Background technique [0002] Fine-bred livestock with excellent traits has always been an important resource in animal husbandry, but the cultivation of fine traits is a long process, and there are still many uncertain factors. Through transgenic technology, the excellent traits of fine-bred livestock can be cultivated in a directional and rapid manner. In August 2006, the world's first human drug recombinant protein produced by transgenic goat mammary gland bioreactor was launched in Europe, which marked that transgenic animals can be used for human medicine. Human service. At present, countries all over the world are increasing investment and research and development efforts, striving to take the lead in the fierce international competition of genetically modified breeding technology, and China has also lau...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85A01K67/027
Inventor 周平王立民王新华刘守仁唐红代蓉
Owner XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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