Expression vector, and its construction and application

Abstract

The invention discloses an expression vector, and its construction and application. The construction of the expression vector comprises adding LoxP sequences at two ends of an untargeted gene of a linear vector and introducing inducible expression Cre enzyme and TK suicide gene components simultaneously. The expression vector improves securities of transgenic animals.

Description

technical field [0001] The invention relates to the technical field of molecular genetics, in particular to an expression vector and its construction and application. Background technique [0002] Fine-bred livestock with excellent traits has always been an important resource in animal husbandry, but the cultivation of fine traits is a long process, and there are still many uncertain factors. Through transgenic technology, the excellent traits of fine-bred livestock can be cultivated in a directional and rapid manner. In August 2006, the world's first human drug recombinant protein produced by transgenic goat mammary gland bioreactor was launched in Europe, which marked that transgenic animals can be used for human medicine. Human service. At present, countries all over the world are increasing investment and research and development efforts, striving to take the lead in the fierce international competition of genetically modified breeding technology, and China has also lau...

AI Technical Summary

Problems solved by technology

[0005] It can be seen that the above-mentioned existing expression vectors obviously still have inconvenience and defects in structure and use, and need to be further improved urgently.

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