Induction technology of anabaena flosaquae chlamydospore and preparation method of dry algae powder thereof

A technology of blooming Anabaena and thick-walled spores, applied in the direction of using spores, biochemical equipment and methods, organic fertilizers, etc., to achieve great theoretical value and application value, simple drying method, and the effect of increasing content

Inactive Publication Date: 2013-01-09
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
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Problems solved by technology

[0007] The present invention aims at the above-mentioned deficiencies existing in the prior art, and provides a kind of induction technology of Anabaena algae thick-walled spores and a preparation method of dry algae powder, aiming at promoting low-carbon agriculture vigorously at present, reducing the application of chemical fertilizers and pesticides, and improving The amount of organic fertilizer and the policy of fertilizer efficiency, and the high water content of the bloom cyanobacteria, the large occupation volume, the disadvantages that are not convenient for transportation and storage, and the defects that the bloom anabaena is not easy to harvest, the invention provides a kind of water bloom Cyanobacteria as raw material, plus physical and chemical conditions to induce a large number of thick-walled spores to form and use plastic film to prepare dry algae powder is an efficient, safe and convenient fertilizer production technology

Method used

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  • Induction technology of anabaena flosaquae chlamydospore and preparation method of dry algae powder thereof
  • Induction technology of anabaena flosaquae chlamydospore and preparation method of dry algae powder thereof
  • Induction technology of anabaena flosaquae chlamydospore and preparation method of dry algae powder thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Main materials: sodium hydroxide, hydrochloric acid, sucrose, culture medium, dry algae powder, plastic film, large porcelain plate.

[0028] Such as figure 1 As shown, the specific plan is to weigh about 0.01g of dried algae powder in bloom, dissolve it in a small beaker containing 10mL of culture solution, and smash it with an algae maker sterilized with 75% alcohol for 3 times, each time for 10s, and then put the The algae solution was transferred to a small petri dish of 90×15mm, the beaker was rinsed with culture solution and then poured into the culture dish, and finally the culture dish was supplemented with culture solution to 35mL. Adjust the pH to 6 with sodium hydroxide and hydrochloric acid, then add 3mg of sucrose to the petri dish, and finally place it in a sterile room with a light intensity of 3000±200Lx and a temperature of 30±2°C. Generally, after 3 days of culture, the content of chlamydospores will reach the peak value. At this time, pour out the ex...

Embodiment 2

[0032] Main materials: sodium hydroxide, hydrochloric acid, glucose, culture medium, dry algae powder, plastic film, large porcelain plate.

[0033] The specific plan is to weigh about 0.01g of dried algae powder in bloom, dissolve it in a small beaker containing 10mL of culture solution, smash it with an algae maker sterilized with 75% alcohol for 3 times, each time for 10s, and then transfer the algae solution Put it into a small petri dish of 90×15mm, rinse the beaker with the culture solution and pour it into the culture dish, and finally add the culture solution to the culture dish to 35mL. Use sodium hydroxide and hydrochloric acid to adjust the pH to 6, then add 3 mg of glucose to the petri dish, and finally place it in a sterile room with a light intensity of 3000±200Lx and a temperature of 30±2°C. Generally, after 3 days of culture, the content of chlamydospores will reach the peak value. At this time, pour out the excess culture medium and keep Anabaena blooms. Then...

Embodiment 3

[0036] Main materials: sodium hydroxide, hydrochloric acid, glucose, culture solution, dry algae powder, plastic film, large petri dish, oven.

[0037] The specific plan is to weigh about 0.01g of dried algae powder in bloom, dissolve it in a small beaker containing 10mL of culture solution, smash it with an algae maker sterilized with 75% alcohol for 3 times, each time for 10s, and then transfer the algae solution Put it into a small petri dish of 90×15mm, rinse the beaker with the culture solution and pour it into the culture dish, and finally add the culture solution to the culture dish to 35mL. Use sodium hydroxide and hydrochloric acid to adjust the pH to 7, then add 3 mg of glucose to the petri dish, and finally place it in a sterile room with a light intensity of 3000±200Lx and a temperature of 30±2°C. Generally, after 3 days of culture, the content of chlamydospores will reach the peak value. At this time, pour out the excess culture medium and keep Anabaena blooms. T...

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Abstract

The invention relates to an induction method of anabaena flosaquae chlamydospore in the field of biotechnologies. The induction method comprises the following steps of: culturing the anabaena flosaquae for 3-4 days under the conditions of adding soil extract, cane sugar or glucose to a liquid culture medium containing dipotassium phosphate, ferric citrate, molybdic acid and calcium carbonate and regulating pH to be 6-7 to realize induction of the chlamydospore; and laying the anabaena flosaquae on a porcelain disc or a big culture vessel of 150*15mm, placing the porcelain disc or the big culture vessel into an oven and baking or placing the porcelain disc or the big culture vessel into a disinfection chamber of 30 DEG C and drying for 3-5 days to obtain dry algae powder. In the invention,the chlamydospore content is greatly improved because of effective and reasonable conditions, the dry algae powder can be obtained more efficiently through a simple drying mode, the method for preparing the dry algae powder with bloom cyanobacteria as a raw material is simple in process, the application of fertilizer is safe and efficient, the method is easy for popularization and implementation,and after the fertilizer is utilized, new pollution on environment and food safety is not brought about, therefore, the induction method of the anabaena flosaquae chlamydospore has great theoretical value and application value.

Description

technical field [0001] The invention relates to an optimized production method in the field of biotechnology, in particular to a technique for inducing thick-walled spores of Anabaena algae blooms and a method for preparing dry algae powder. Background technique [0002] Anabaena flos-aquae (Anabaena flos-aquae) is provided by the Freshwater Algae Species Bank of the Institute of Hydrobiology, Chinese Academy of Sciences (No.: FACHB-245), recorded in "Jianying Shen, Antomo DiTommaso, Mingquan Shen, Wei Lu, and Zhengming Li; Molecular basis for differential metabolic responses to monosulfuron inthree nitrogen-fixing cyanobacteria, Weed Science, 2009, 57: 178-188", "Jianying Shen, JingJiang, Peizhong Zheng; Effects of Light and Monosulfuron on growth and Photosynthetic Pigena fof An , Water Resource and Protection, 2009, 1: 408-413", "Zheng Peizhong, Shen Jianying; Research on the Effect of Organic Solvent Acetone on the Growth of Nitrogen-fixing Cyanobacteria, Shanghai Agricu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N3/00C05F11/08
Inventor 沈健英万旗东郑培忠陈瑞
Owner SHANGHAI JIAO TONG UNIV
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