Salmonella/Shigella dual fluorescent PCR detection kit

Abstract

The invention discloses a salmonella and shigella dual fluorescence PCR (Polymerase Chain Reaction) detection kit which comprises the following components of PCR reaction liquid, salmonella and shigella dual reaction liquid and sterilized water. According to the salmonella and shigella dual fluorescence PCR (Polymerase Chain Reaction) detection kit disclosed by the invention, the problems of complicated operation, longer cycle and incapability of simultaneously detecting in a reaction and the like in the detection of the salmonella and shigella by using the traditional biochemical and immunological method are solved; the salmonella and the shigella can be simultaneously detected in the same reaction tube; moreover, the salmonella and shigella dual fluorescence PCR (Polymerase Chain Reaction) detection kit has the advantages of strong specificity, high sensitivity, convenience for operation, strong repeatability, repaid and objective detection result and the like.

Description

technical field [0001] The invention relates to an in vitro diagnostic kit for intestinal bacteria, in particular to a dual fluorescent PCR detection kit capable of simultaneously detecting Salmonella and Shigella, belonging to the field of in vitro diagnosis of Salmonella and / or Shigella. Background technique [0002] Salmonella is a kind of Gram-negative intestinal pathogenic bacteria widely distributed in nature, which can cause great harm to humans and animals, and can cause symptoms such as gastroenteritis, typhoid fever, and sepsis in humans. Salmonella is a genus of Enterobacteriaceae. This genus of bacteria is Gram-negative short bacilli without spores. Except for Salmonella pullorum and Salmonella gallinarum typhi, all other pathogenic bacteria of livestock and poultry have peripheral flagella, and most of them have pili. Adsorbed on the surface of red blood cells, it can agglutinate red blood cells. Since Salmonella is a group of Gram-negative bacteria with simila...

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Problems solved by technology

[0011] The technical problem mainly solved by the present invention is to overcome the defects such as low sensitivity and poor specificity of existing Salmonella and/or Shigella in vitro diagnostic kits

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