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Banksia culture medium as well as tissue culture and fast propagation method

A tissue culture and culture medium technology, applied in the field of medium for plant tissue culture, can solve the problems such as the medium that has not yet been found in the medium, the rapid propagation of B. The effect of keeping the excellent seed properties unchanged, improving the quality of seedlings and shortening the period of seedlings

Active Publication Date: 2013-08-28
BEIJING FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] If the method of tissue culture is used to propagate Bankwood, due to the special growth environment of the plant, the demand for temperature, light and nutrient elements is high. Too high temperature or too low light intensity will lead to poor growth of tissue culture seedlings.
So far, no medium suitable for Bankia has been found, and there has been no literature report using tissue culture methods to rapidly propagate Bankia, especially the subculture of its seedlings, induction of axillary buds and rooting

Method used

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  • Banksia culture medium as well as tissue culture and fast propagation method
  • Banksia culture medium as well as tissue culture and fast propagation method
  • Banksia culture medium as well as tissue culture and fast propagation method

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Effect test

Embodiment 1

[0040] To prepare No. 1 modified WPM medium, the macroelement components used include: NH 4 NO 3 , 400mg / L; Ca(NO 3 ) 2 4H 2 O, 556mg / L; KCl, 894.5mg / L; CaCl 2 2H 2 O, 192mg / L; MgSO 4 , 370mg / L.

[0041] To prepare No. 2 modified WPM medium, the macroelement components used include: NH 4 NO 3 , 200mg / L; Ca(NO 3 ) 2 4H 2 O, 278mg / L; KCl, 447.25mg / L; CaCl 2 2H 2 O, 96mg / L; MgSO 4 , 185mg / L.

[0042] The lighting conditions are 2500-3000Lux of natural scattered light plus 2000-2500Lux of artificial auxiliary light, and the lighting time is 14h / d. The light conditions and light time are applied to each culture stage of Bankwood tissue culture (except that the disinfection of explants does not require light).

[0043] The culture temperature of Bankwood tissue culture is 20-23°C, which is applied to the following aseptic seedling acquisition, axillary bud induction, subculture and rooting culture stage; the transplanting temperature of test-tube seedling transplanti...

Embodiment 2

[0056] To prepare No. 1 modified WPM medium, the macroelement components used include: NH 4 NO 3 , 400mg / L; Ca(NO 3 ) 2 4H 2 O, 556mg / L; KCl, 894.5mg / L; CaCl 2 2H 2 O, 192mg / L; MgSO 4 , 370mg / L.

[0057] To prepare No. 2 modified WPM medium, the macroelement components used include: NH 4 NO 3 , 200mg / L; Ca(NO 3 ) 24 h 2 O, 278mg / L; KCl, 447.25mg / L; CaCl 22 h 2 O, 96mg / L; MgSO 4 , 185mg / L.

[0058] The cultivation light condition is 2500-3000Lux of natural scattered light plus 2000-2500Lux of artificial auxiliary light, and the light time is 14h / d. The light conditions and light time are applied to each culture stage of Bankwood tissue culture (except that the disinfection of explants does not require light).

[0059] The culture temperature of Bankwood tissue culture is 20-23°C, which is applied to the following aseptic seedling acquisition, axillary bud induction, subculture and rooting culture stage; the transplanting temperature of test-tube seedling transpl...

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Abstract

The invention provides a culture medium and a tissue culture and fast propagation method for Banksia. The special culture medium for the Banksia is an improved WPM (Woody Plant Medium) consisting of the following elements: 200-400mg / L of NH4NO3, 250-600mg / L of Ca(NO3)24H20, 400-900mg / L of KCl, 90-200mg / L of CaCl22H2O and 180-400mg / L of MgSO4. The tissue culture and fast propagation method for theBanksia comprises the following steps of sterilizing explants, obtaining aseptic seedlings, inducing axillary buds, carrying out subculture, rooting and transplanting seedlings in test tubes. According to the tissue culture and fast propagation method for the Banksia, disclosed by the invention, the propagation coefficient and rooting rate of the Banksia are largely increased, a set of complete fast propagation technical system is established, and the technical support is provided for the wide popularization and application of the Banksia.

Description

technical field [0001] The invention relates to a culture medium for plant tissue culture, in particular to a culture medium for bankoyakia, and a tissue culture and rapid propagation method for bankoyakia. Background technique [0002] Bankwood is a shrub or small tree belonging to the family Longanaceae. It is mainly distributed in the southeastern and southwestern coastal areas of Australia. It is suitable for growing on sandy soil, sandy loam soil or sandy sandstone. It is a new type of woody flower, and its leaves, flowers and cones are all excellent materials for making dried flowers. Regarding the propagation methods of Bankwood, at present, they are mainly seed propagation and cutting propagation, but due to the limitation of seasons and climatic conditions, the propagation coefficient is very low, and it cannot be widely promoted in production under the condition of limited resources. Tissue culture is an important way to rapidly propagate fine plant varieties, esp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04
Inventor 潘会堂马琳张启翔王佳程堂仁孙明
Owner BEIJING FORESTRY UNIVERSITY
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