Method for preparing taxillus chinensis danser extract and application
A technology of duck foot veneer and extract, which is applied in the field of research and development of pharmaceutical products, can solve the problems that the research on the anti-inflammatory and analgesic effects of duck foot veneer extract has not been reported yet, achieve significant anti-inflammatory and analgesic activity, and the preparation method is simple and Appropriate effect for promoting the application
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Embodiment 1
[0022] The preparation of embodiment 1 duck foot veneer extract
[0023] (1) Get the bark powder of Schefflera octophylla (Lour.) Harms (the particle size of the powder is not strictly limited), and use 10 times the volume ratio concentration of 95% ethanol to reflux extract 3 times, each time for 2 hours , static filtration;
[0024] (2) Merge step (1) gained filtrate, reclaim solvent under reduced pressure and obtain total extract;
[0025] (3) Suspend the total extract obtained in step (2) with an appropriate amount of water (the amount of water is about 3 to 5 times the mass of the total extract), and then sequentially extract each extract with petroleum ether, chloroform, ethyl acetate and n-butanol. 3 times, the amount of extractant is 1 to 2 times that of the aqueous solution;
[0026] (4) merging step (3) gained sherwood oil layer, chloroform layer, ethyl acetate layer and n-butanol layer extract respectively;
[0027] (5) The obtained petroleum ether layer, chlorof...
Embodiment 2
[0028] The preparation of embodiment 2 duck foot veneer extract
[0029] (1) Take the bark powder of Schefflera octophylla (Lour.) Harms), extract it twice with 85% ethanol at a concentration of 8 times the volume ratio, and extract it twice for 2.5 hours each time, and let it stand and filter;
[0030] (2) Merge step (1) gained filtrate, reclaim solvent under reduced pressure and obtain total extract;
[0031] (3) Suspend the total extract obtained in step (2) with water (the amount of water is about 3 to 5 times the mass of the total extract), and then sequentially extract each 4 times with petroleum ether, chloroform, ethyl acetate and n-butanol ; (extractant consumption is 1~2 times of aqueous solution)
[0032] (4) merging step (3) gained sherwood oil layer, chloroform layer, ethyl acetate layer and n-butanol layer extract respectively;
[0033] (5) The obtained petroleum ether layer, chloroform layer, ethyl acetate layer and n-butanol layer extract and the water layer ...
Embodiment 3
[0034] The preparation of embodiment 3 duck foot veneer extract
[0035] (1) Take the bark powder of Schefflera octophylla (Lour.) Harms, extract it with 9 times volume ratio concentration of 75% ethanol under reflux for 3 times, each time for 2 hours, let stand and filter;
[0036] (2) Merge step (1) gained filtrate, reclaim solvent under reduced pressure and obtain total extract;
[0037] (3) Suspend the total extract obtained in step (2) with water (the amount of water is about 3 to 5 times the mass of the total extract), and then sequentially extract each 4 times with petroleum ether, chloroform, ethyl acetate and n-butanol ; (extractant consumption is 1~2 times of aqueous solution)
[0038] (4) merging step (3) gained sherwood oil layer, chloroform layer, ethyl acetate layer and n-butanol layer extract respectively;
[0039] (5) The obtained petroleum ether layer, chloroform layer, ethyl acetate layer and n-butanol layer extract and the water layer after the extraction ...
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