Application of perylenequinone compound as feed coloring agent
A technology of compounds and colorants, applied in the direction of animal feed, animal feed, application, etc., can solve the problems of low nutritional value, reduced coloring effect, instability, etc.
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Embodiment 1
[0053] Embodiment 1: acute toxicity test
[0054] The perylenequinone compound was purified by preparative chromatography to obtain crystals of Calphostin C, Cercosporin, Cladosporium A, Elsinochrome C, Hypocrellin A, Hypocrellin B, Phleichrome, Scutiaquinone A with a purity of about 97%, and then used for acute toxicity tests on mice and chicks respectively
[0055] (1) Acute toxicity test in mice
[0056] Select 120 mice in good body condition, healthy and similar in weight, half male and half female, and randomly divided into 6 groups, 20 mice in each group. Experiment 1 group was the control group, fed with distilled water. On the basis of the preliminary test, the feeding doses of the five administration groups were 0.1, 0.2, 0.3, 0.4, 0.5 g / kg respectively, and they were given orally with a metal gastric tube after fasting for 18 hours.
[0057] During the test, the toxic reaction, morbidity and death of the mice were observed and recorded. After the end of the 7-day ...
Embodiment 2
[0067] Embodiment 2: subacute toxicity test
[0068] The perylenequinone compound was purified by preparative chromatography to obtain crystals of Calphostin C, Cercosporin, Cladosporium A, Elsinochrome C, Hypocrellin A, Hypocrellin B, Phleichrome, and Scutiaquinone A with a purity of about 97%, and then used for subacute toxicity in mice and chicks respectively test
[0069] (1) Subacute toxicity test on mice
[0070] Select 80 mice in good body condition, healthy and with similar body weight, half male and half female, and randomly divided into 4 groups, 20 mice in each group, including 3 drug administration groups and 1 control group. On the basis of acute toxicity test and clinical verification test, three dosage groups of high dose (0.04 g / kg), medium dose (0.01 g / kg) and low dose (0.002 g / kg) were set up. The tube was orally administered once a day for 14 consecutive days. During the experiment, the mice took food and water by themselves and kept the environment clean ...
Embodiment 3
[0113] Example 3: Experiment of adding solid-state fermented hypocrellin to chicken feed as a coloring agent
[0114] The basic dietary formula for chickens in the experiment is: white corn 60%, millet 5%, sorghum 4.2%, wheat bran 5%, bean cake 8%, fish meal 8%, meat and bone meal 3%, blood meal 5%, stone powder 1.5%, salt 0.3%. The basic daily ration was powder, which was crushed with a 1.8 mm sieve.
[0115] Hypocretin is produced by solid-state fermentation, corn is the main raw material, and 1% glucose and 0.3% KH are added based on the weight of corn 2 PO 4 , 0.02% MgSO 4 ·7H 2 O. Add water and mix the material to make it wet, the ratio of material to water is controlled at 1:0.7, sterilize at 121°C for 45-60 minutes, and then add 10% liquid Shiraia sp. SUPER-H168 seeds after cooling. Both the initial pH and the pH of the fermentation process are natural, the fermentation temperature is 30°C, the humidity is controlled at 90% during the fermentation process, and the...
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