Two-cutting embryo taking method for young grape fruits
A technology for young fruit and grapes, which is applied in the field of embryo extraction from young grape fruit and horticultural biology. It can solve the problems of low embryo extraction efficiency, easy pollution, low ovule survival rate and ovule development rate, etc., to improve efficiency, save resources, The effect of high ovule survival rate and development rate
Inactive Publication Date: 2013-04-03
HORTICULTURE INST OF XINJIANG ACAD OF AGRI SCI
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Problems solved by technology
[0003] The main purpose of the present invention is to address the low ovule survival rate and ovule development rate existing in the current traditional grape embryo rescue technology using the grape aseptic embryo stripping method, as well as the low efficiency of embryo extraction and the technical status quo that is easy to pollute
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Experimental program
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Effect test
Embodiment
[0018] Disinfect the young grape fruit with 75% ethanol in the aseptic operation table, disinfect both hands, grasp the fruit stem and the base of the fruit with the left hand, and hold the scalpel in the right hand to make a horizontal cut from the middle part near the embryo to about 2 / 5 of the fruit grain. The circle should be deep enough not to hurt the embryo. Gently break it apart with your hands to see clearly the place where the embryo is born. The second knife cuts vertically into two halves, and finally sticks or picks out the ovule with the tip of the knife.
[0019] The present invention is slightly inconvenient because it is operated in an aseptic platform. This method is suitable for taking embryos before the grape fruit turns color and becomes soft. It has the characteristics of no pollution, high ovule survival rate and development rate, and generally improves the efficiency. , saving resources. The two-knife embryo extraction method for grape young fruit can a...
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The invention discloses a two-cutting embryo taking method for young grape fruits. In a sterile operation table, the young grape fruit is sterilized by 75% ethanol; both hands are sterilized, the left hand tightly holds a carpopodium and a fruit stem base, and the right hand holds a scalpel to carry out transverse cutting for one circle from the middle about 2 / 5 position of a fruit grain near oneside of the embryo, for the first cutting; the depth is not deep enough to hurt the embryo; the young grape fruit is lightly torn to clearly see the embryo insertion position; the second cutting is characterized by vertically cutting one time in half from the middle; and finally, an ovule is soaked out or picked out with a knife point. The method provided by the invention is suitable for taking the embryo before the grape fruit grain changes color and becomes soft, the pericarp instead of the young embryo is directly disinfected, and the method has the characteristics of no pollution, high ovule survival rate and high developmental rate. Efficiency is improved, resource is saved, and the method has wide applicability.
Description
field of invention [0001] The invention relates to the technical field of horticultural biology. Specifically, the present invention relates to the technical field of extracting embryos from grape young fruit. Background technique [0002] In the traditional grape embryo rescue technology, there are two methods for the extraction of sterile grape embryos. One is to pick the fruit pieces, rinse them with tap water, peel off the ovules with sterile tweezers and a scalpel, place them in a sterile operating table, disinfect the ovules with 70% ethanol, rinse them with sterile water, and then use 0.1% Sterilized with mercuric chloride and rinsed with sterile water, the aseptic embryos were obtained, but the ovule survival rate and ovule development rate were low. The second is to pick the young fruit, wash it with tap water, soak it with 70% ethanol, rinse it once with sterile water, pour 0.1% mercury liter on the ultra-clean workbench to soak and shake it several times, and th...
Claims
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IPC IPC(8): A01H4/00
Inventor 伍新宇罗淑萍梅新娣夏培蓓潘明启
Owner HORTICULTURE INST OF XINJIANG ACAD OF AGRI SCI