Method and kit for sequencing target areas

Abstract

The invention relates to the field of genetic engineering, and provides a method and a kit for sequencing target areas. The method comprises the following steps of: A, using a specificity primer to amplify a plurality of target areas in a sample to be detected, and establishing a sequencing library based on amplified products; B, subjecting the sequencing library to monomolecular amplification to obtain a plurality of monomolecular amplification products corresponding to the plurality of target areas; and C, subjecting the plurality of monomolecular amplification products to high-throughput gene sequencing at the same time so as to obtain sequence information of the plurality of target areas. The method and the corresponding kit are used for sequencing the plurality of areas at the same time by using a high-throughput sequencing technology; the sequence information including variation of known mutations and unknown mutations in the areas can be obtained accurately; the kit has high detection sensitivity, and can be used for further detecting mainly samples at the same time.

Description

technical field [0001] The invention relates to the field of genetic engineering, more specifically, to a method and a kit for sequencing a target region. Background technique [0002] Since the advent of the draft nucleotide sequence of the human genome and many other draft genomes, as well as the discovery of a large number of single nucleotide polymorphisms (SNPs) and small insertion / deletion polymorphisms in the draft genomes of humans and many other organisms, people began to Interested in nucleic acid determination. Studies have shown that nucleic acid sequence variations at specific positions contain a large amount of biological information, which may be related to certain traits of humans or animals and plants. These information will have a wide impact on all aspects of medicine and agriculture. For example, it is anticipated that developments in this field may enable personalized medicine. [0003] At present, the common method for sequencing the target region (sp...

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Problems solved by technology

[0003] At present, the common method for sequencing the target region (specific position) is the Sanger sequencing method. The Sanger sequencing method can detect the target region, but the Sanger sequencing method can only sequence a certain region of a sample at a time. High cost, low sensitivity (20%), and accurate data on the variation of each mutation site cannot be obtained

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