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Strain produced by beta-glucosidase and method for preparing Genipin therefrom

A technology for producing glucosidase and bacteria, applied in the biological field, can solve problems such as limited development, high cost, and complicated process, and achieve the effects of reducing production costs, increasing production, and improving production efficiency

Active Publication Date: 2013-07-17
TIANJIN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at the same time, there are many shortcomings, such as: complicated process and high cost, which limit its further development

Method used

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  • Strain produced by beta-glucosidase and method for preparing Genipin therefrom
  • Strain produced by beta-glucosidase and method for preparing Genipin therefrom
  • Strain produced by beta-glucosidase and method for preparing Genipin therefrom

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Embodiment Construction

[0022] Below in conjunction with the examples, the present invention is further described, the following examples are illustrative, not limiting, and the protection scope of the present invention cannot be limited by the following examples.

[0023] 1. Determination of soil

[0024] The soil samples were taken from the soil of cattle manure accumulated for many years in the Wuqing cattle farm in Tianjin. The 1% mastigma extract was used as the substrate for the enzyme reaction, and the potato medium formula was used to form the primary screening medium. After hydrolysis by glucosidase, genipin is produced and reacted with glycine to form blue color to judge whether there is a fungal strain producing β-glucosidase. The process is as follows:

[0025] (1) Preliminary screening of strains: After the soil is pretreated, take 1 mL of the soil and put it into a (25 mL / 250 mL) enrichment medium for culture, at 28 °C, 180 r / min, and culture for 36 hours;

[0026] The composition of t...

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Abstract

The invention relates to a method for effectively converting Geniposide into Genipin by stimulating the conversion of microbes in the in vivo intestinal tract through an in vitro microbial fermentation process. The method is characterized in that: a traditional Chinese medicine cape jasmine is fermented by the high yield strain of beta-glucosidase screened and preserved in a laboratory of the inventors, and an optimum condition for obtaining the product Genipin through the microbial conversion is obtained by optimizing through single factor experiments. According to the method of the invention, Geniposide in Genipin in hydrolyzed under mild conditions, so a defect of the effective component loss of traditional methods is avoided; and the scale conversion of the effective component Geniposide of cape jasmine is realized under in vitro controllable conditions with a modern microbial fermentation engineering technology, so the method of the invention has large economic benefits and social benefits, and has a wide market exploitation prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to microbial transformation technology, in particular to a β-glucosidase producing bacterium and a method for preparing genipin by transforming the bacterium. Background technique [0002] Genipin is a kind of iridoid substance, non-toxic, easily soluble in organic solvents such as ethanol, acetone, ethyl acetate, and less soluble in water. In recent years, due to the remarkable curative effect of genipin in anti-tumor and treatment of liver cirrhosis, and as an emerging drug intermediate with many new and important pharmacological values, it has a good application prospect in the development of injections, etc. . At the same time, genipin is also used in biological materials as a new type of biological cross-linking agent. It can not only form stable cross-linked products, but also has low cytotoxicity, good biocompatibility, strong resistance to degradation and wide application fields....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P17/06C12R1/685
Inventor 李玉荆玮刘逸寒刘晓光王稳航路福平
Owner TIANJIN UNIV OF SCI & TECH
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