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Phytase microbial inoculum and preparation method thereof

A microbial agent and a technology of microbial agents, applied in the field of phytase microbial agents, can solve the problems of accelerating the depletion of mineral resources, increasing the incidence of diseases and insect pests, and reducing the quality of agricultural products, achieving considerable economic and social benefits, improving quality, The effect of promoting growth and development

Inactive Publication Date: 2012-03-21
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Reduce the quality of agricultural products and increase the incidence of diseases and insect pests
At the same time, the extraordinary development of fertilizer production has accelerated the depletion of mineral resources

Method used

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  • Phytase microbial inoculum and preparation method thereof
  • Phytase microbial inoculum and preparation method thereof
  • Phytase microbial inoculum and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The obtaining method one of embodiment 1 phytase microbial bacterium preparation

[0016] Pick an inoculated loop Bacillus Lip-3 thallus (screened and collected by this experiment) from the bacterial classification of the slant medium (0.3% beef extract, peptone 1%, NaCl 0.5%, agar powder 1.5%, pH 7.0~7.2.) Stored and sold) into 50ml of liquid medium (0.3% beef extract, 1% peptone, 0.5% NaCl, pH 7.0-7.2), cultured with shaking at 37°C for 12h, which is the seed solution. Add 300ml medium (0.3% beef extract, 1% peptone, 10.5% NaC room, pH 7.0-7.2) to a 500ml Erlenmeyer flask, insert the seed liquid into the fermentation medium with 10% inoculum size, and culture with shaking at 37°C. Obtain phytase microbial bacterial agent.

Embodiment 2

[0017] The obtaining method two of embodiment 2 phytase microbial preparations

[0018] The seed liquid of Bacillus Lip-3 bacteria was inoculated into the fermentation medium with 10% inoculation amount, and cultured in a fermenter at 30°C for 4 days with aeration. Obtain phytase microbial bacterial agent.

Embodiment 3

[0019] The fertilizer of embodiment 3 phytase microbial bacterium preparations

[0020] The Bacillus Lip-3 bacterium cultured by aeration in the fermenter was mixed with chicken manure and crop stalks. Mixing ratio: Bacillus Lip-3 cell fermentation liquid 10%, chicken manure 50%, crop straw 40%. Solid fermentation 30d. Get phytase biofertilizer.

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Abstract

The invention relates to a phytase microbial inoculum which comprises a Bacillus Lip-3 strain and a fermentation culture medium, wherein the concentration of the Bacillus Lip-3 strain is 2-10*10<8> / mL; and the fermentation culture medium comprises the following components in percentage: 0.3-0.5% of beef extract, 0.5-1.5% of peptone and 0.4-0.6% of NaCl. The phytase microbial inoculum can promote the plant growth, increase the crop yield and improve the quality. The phytase microbial inoculum can be used for the cultivation of various vegetables, melons and fruits in agricultural greenhouses, such as cucumbers, tomatoes, pakchoi, kidney beans, aubergines, romaine lettuces, leaf lettuces, towel gourds, peppers, rape, sweet melons, strawberries, grapes and the like; the phytase microbial inoculum can be used for field crops, such as corn, wheat, soybeans, sugarcanes and the like; and the phytase microbial inoculum can be also used for fruit trees, such as peach trees, pear trees, jujube trees, apple trees and the like. Especially, the phytase microbial inoculum can promote the growth of Brassica chinensis L., can obviously increase the yield of cucumbers and tomatoes, and has considerable economic and social benefits.

Description

technical field [0001] The invention relates to a phytase microbial preparation. Background technique [0002] Increasing the number of beneficial microorganisms in the soil can improve soil fertility, as described in the published book "Soil Fertilily" by Liu Lili et al. (Nova Press, 2009: New York). The method of increasing soil fertility by increasing the number of beneficial microorganisms in the soil is a method of developing green ecological agriculture, and it is a method that our country should vigorously develop and support. Because my country is the country that uses the largest amount of chemical fertilizers and pesticides in the world. Extensive application of chemical fertilizers has not only caused soil pollution, but also aggravated the eutrophication of water bodies, and even affected groundwater and air. Excessive application of chemical fertilizers also destroys the soil structure of cultivated land. Reduce the quality of agricultural products and increa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08A01N63/00A01P21/00C12R1/07
Inventor 刘丽丽陈立新
Owner TIANJIN NORMAL UNIVERSITY
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