Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for extracting coenzyme Q10 from tobaccos

A tobacco and coenzyme technology, applied in organic chemistry, quinone separation/purification, etc., can solve problems such as cumbersome operation steps, complex chemical components, and long extraction time

Active Publication Date: 2014-02-12
NORTHEAST FORESTRY UNIVERSITY +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But since coenzyme Q 10 It is a heat-sensitive substance, and the chemical composition in tobacco is complex, the conventional method takes a long time to extract, and the operation steps are cumbersome, which affects the coenzyme Q. 10 The reliability of the extraction speed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take 10 kg of tobacco suspension culture cells separated from the culture medium and 10 liters of 75% ethanol-petroleum ether solution (the volume ratio of 75% ethanol and petroleum ether is 2:1), mix and homogenate for 5 minutes, and after the homogenization is completed, the above-mentioned The homogenate mixture is filtered, allowed to stand for stratification, and the upper layer solution is concentrated to obtain coenzyme Q 10 Crude extract of Coenzyme Q 10 The content in the crude extract is 1.2%.

Embodiment 2

[0011] Get 10 kilograms of tobacco suspension culture tissue pieces that have been separated from the culture medium and 10 liters of 85% ethanol-petroleum ether solution (the volume ratio of 75% ethanol and petroleum ether is 1: 1), mix and homogenate for 10 minutes, after the homogenization is finished, The above homogenate mixture is filtered, allowed to stand for stratification, and the upper layer solution is concentrated to obtain coenzyme Q 10 Crude extract of Coenzyme Q 10 The content in the crude extract is 1.6%.

Embodiment 3

[0013] Get 10 kilograms of tobacco suspension culture tissue pieces that have been separated from the culture medium and 10 liters of 80% ethanol-petroleum ether solution (the volume ratio of 75% ethanol and petroleum ether is 1:2), mix and homogenate for 8 minutes, after the homogenization is finished, The above homogenate mixture is filtered, allowed to stand for stratification, and the upper layer solution is concentrated to obtain coenzyme Q 10 Crude extract of Coenzyme Q 10 The content in the crude extract is 1.3%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the chemical field of natural products, in particular to a method for extracting coenzyme Q10 from tobaccos. The method comprises the following processes of: separating tobacco suspension culture cells or tobacco suspension culture tissue blocks from culture solution, and mixing with 75 to 85 percent of ethanol-petroleum ether, homogenizing, filtering the homogenized mixture, standing for demixing, and concentrating supernatant to obtain a crude extract of the coenzyme Q10.

Description

Technical field: [0001] The invention relates to the field of natural product chemistry, in particular to a method for extracting coenzyme Q from tobacco 10 Methods. Background technique: [0002] coenzyme Q 10 It is a fat-soluble ubiquinone compound, the chemical name is 2,3-dimethoxy-5-methyl-6-decenylbenzoquinone, and its quinone ring plays a role in the transfer of electrons and protons in the oxidative respiratory chain important role. coenzyme Q 10 It has obvious effects in the treatment of diseases such as cardiovascular system diseases, cancer and atherosclerosis. In recent years, domestic scholars have also carried out some tobacco cell suspension culture and tobacco leaf tissue block suspension culture to produce coenzyme Q 10 The results showed that the production of coenzyme Q with tobacco as raw material 10 Has good prospects. [0003] For example, on June 4, 2003, the Patent Office of the State Intellectual Property Office of the People's Republic of Chi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07C50/28C07C46/10
Inventor 李春英赵春建祖元刚付玉杰
Owner NORTHEAST FORESTRY UNIVERSITY